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| Effect of cardiac fibroblasts on cardiomyocyte hypertrophy induced by high glucose |
| ZHAO Long, LIU Ling-ling, LI Xiao-li, ZHOU Yan-fang, WANG Hao, ZHANG Ying-yu，
KONG Biao,SHEN Dong-li, ZHANG Guo-hui |
| (Department of Cardiology,the Affiliated People′s Hospital of Jiangsu University, Zhenjiang
Jiangsu 212002,China) |
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Abstract Objective: To investigate the effect of cardiac fibroblast on cardiomyocyte hypertrophy induced by high glucose. Methods: Neonatal CFs and CMs isolated from 0to 3dayold Sprague-Dawley rats were cultured in vitro,then randomly divided into six groups:CM low glucose group（group A）,CM high glucose group（group B）,CM and CF co-culture high glucose group（group C）,CM-CF co-culture + TGF-β1 neutralizing antibody high glucose group（group D）,CF low glucose group（group E）,CF high glucose group（group F）.After cultured different times (0,6,12,24,48,72),cellular morphologies were observed under the inverted phase contrast microscope, the mRNA levels of cardiomyocyte hypertrophy markers ANP、β-MHC were determinated by RT-PCR, the expression of TGF-β1 evaluated by ELISA. Results: Compared with group A, cell surface area of group B cultured for 48 h increased significantly (P<0.05), group C cultured for 24 h increased significantly (P<0.05) respectively. RT-PCR showed that compared with group A ,the expression of ANP、β-MHC mRNA of group B and D cultured for 12 h increased significantly(P<0.05) ,cultured for 24 h increased at most, group C cultured for 6 h increased significantly (P<0.05),cultured for 12 h increased at most respectively. ELISA showed that compared with group A ,the expression of TGF-β1 of group B cultured for 12 h increased significantly (P<0.05) ,group C cultured for 6 h increased significantly (P<0.05) respectively；compared with group E ,6 h later group F increased significantly (P<0.05) respectively. Conclusion: Cardiac fibroblastsmay via paracrine transforming growth factor-β1 promote cardiomyocyte hypertrophy induced by high glucose.
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