|
|
Protective effect of lycopene on hepatic fibrosis in mice |
ZHAO Kai |
(Department of Gastroenterology, Jintan Hospital Affiliated to Jiangsu University, Jintan Jiangsu 213200, China) |
|
|
Abstract Objective: To investigate protective effect of lycopene on liver fibrosis in mice induced by concanavalin A (Con A). Methods: Twentyfour mice were equally randomized into 3 groups: control group, model group and prevention group. The mice in model group was received intraperitoneal injection of CoA(20 mg/kg),one time per week, while control group received normal saline, prevention group was received intragastric administration of lycopene, one time per day, for 8 weeks. Mice blood were collected to test the indices of hepatic fibrosis. Grade of hepatic fibrosis were evaluated according to the semi-quantitative scoring system (SSS). Western blotting was used to test the expression of α-smooth muscle actin(α-SMA), Smad3 protein(Smad3) and transforming growth factor-β1 (TGF-β1). Results: Compared with the control group,the model group showed higher serum levels of hyaluronic acid (HA), laminin (LN), procollagen type Ⅲ(PCⅢ) , SSS score, malonaldehyde level, the expression of α-SMA, TGF-β1 and Smad3, lower superoxide dismutase (SOD) (both P<0.01); compared with the model group, the prevention group showed lower serum levels of HA, LN, PCⅢ, SSS score, malonaldehyde level, the expression of α-SMA, TGF-β1 and Smad3, higher levels of SOD (both P<0.01). Conclusion: Lycopene could sharply decrease the harm of peroxidation, protect the liver cells, reduce the collagen production and restrain the expression of the important factor of α-SMA, Smad3, TGF-β1 which lead to liver fibrosis, so that lycopene had the notable alleviate effect on liver fibrosis induced by ConA.
|
Received: 01 May 2014
|
|
|
|
[1]张炜,范钰,朱丽群,等. 番茄红素对小鼠肝纤维化的治疗作用研究\[J\]. 时珍国医国药,2008,19(7):1743-1744.[2]潘红芳,赵凯,张炜. 不同剂量刀豆蛋白A腹腔注射诱导昆明小鼠肝纤维化的效果观察\[J\]. 中国保健营养,2012,23(5):687-690.[3]赵凯,张炜. 番茄红素对Con A诱导的小鼠肝纤维化SOD、MDA的影响\[J\].安徽医药,2012,16(12):1764-1765.[4]李侠,田小朋,黄欢,等. 地顶胞霉菌丝体对免疫性肝纤维化大鼠的保护作用\[J\]. 安徽医药,2012,16(2):152-154.[5]石蕾,尹一然,李昌平. 舒肝颗粒对大鼠肝星状细胞活化增殖的抑制作用\[J\].中国实验方剂学杂志,2012,18(3):136-138.[6]扈彩霞,郑加生,王玉珍,等.大鼠肝纤维化组织内ROCK-I、磷酸化MBS Thr-697和α-SMA表达增强\[J\]. 基础医学与临床,2010,30(4):355-359.[7]郑春素. 加味茵芍散对肝纤维化家兔TGF-β1、Smad2、Smad4 mRNA表达的影响\[J\]. 中华中医药杂志,2012,27(9):2462-2464.[8]高湲,陈桂敏,梁振钰,等. 芪蚣抗纤方中活血化瘀药对肝纤维化大鼠转化生长因子-β1及Smad2/3的影响\[J\].中国实验方剂学杂志,2012,18(15):260-262.[9]党双锁,李亚萍. TGF-β1在肝纤维化研究中的新进展\[J\]. 世界华人消化杂志,2010,18(16):1631-1636.[10]黄越龙,周春光,陈燕,等. TGF/Smad信号通路与肝纤维化的研究进展\[J\].实用医学杂志,2011,27(10):1883-1884.[11]Latella G, Vetuschi A, Sferra R, et al. Targeted disruption of Smad3 confers resistance to the development of dimethylnitrosamine-induced hepatic fibrosis in mice\[J\].Liver Int, 2009, 29(7):997-1009. |
|
|
|