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| Effect of IL-33／TLR4 signal pathway in inducing A549 cells transition to mesenchymal cells from epithelium in vitro |
| LIU Chao, ZHENG Jin-xu, XU Jiao, ZHU Qin, DU Chuan-chong |
| (Department of Respiratory Medicine, the Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001, China) |
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Abstract Objective: To investigate the role and mechanism of IL-33 in the process of turning epithelium-mesenchyme for A549 cells which is in one strain of human alveolar epithelial cells. Methods: To stimulate cultured A549 cells with TGF-β1(5 ng／mL). MTT assay was used to assess the proliferation of the A549 cells in response to TGF-β1 treatment the dynamic expressions of IL-33／TLR4 mRNA were detected by RT-PCR. Western blotting were employed to examine the mRNA and protein expressions of α-smooth muscle actin (α-SMA) and E-cad, p-AKT. Results: The data showed that TGF-β1 had no obvious effects on the proliferation of A549 cells. Stimulated by TGF-β1,E-cad expression of A549 cells gradually declined. αSMA expression was higher. p-AKT expression gradually increased. Real-time PCR had shown that expressions of IL-33／TLR4 mRNA increased firstly then decreased gradually. The significant differences were observed from 0 h to 24 h, especially on 24 h,compared with control group(P<0.05). Conclusion: IL-33／TLR4／PI3K／AKT signal transduction pathway promoted epithelialmesenchymal transition (EMT) of A549, especially on earlier inflammatory reaction.
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Received: 24 March 2014
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2014, Vol. 24 
