大豆异黄酮对动脉粥样硬化大鼠炎症分子相关基因表达的影响

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Abstract Objective: To investigate the effects of soy isoflavones(SIF) on the expression of gene for proinflammatory of atherosclerosis(As) in the As rats. Methods: To establish the rats model of As, were randomly divided into normal control, model, model+SIF and normal+SIF group; the pathological changes of thoracic aorta of rats in each group were observed by HE staining; Real time-PCR and immunohistochemical detection of the expression of gene for proinflammatory of As in vessels. Results: The model group showed severe atrophy and calcification of thoracic aorta compared with normal control group, even appear atherosclerotic plaques; compared with model group, model+SIF group showed actions in lessening the atherosclerosis lesion obviously; both normal control group and normal+SIF group without pathological changes of thoracic aorta. Compared with model group, mRNA level of MCP-1/CCR2, MCPIP and CRP were higher than that in normal control group of thoracic aorta（P<0.01 or P<0.05）; mRNA level of the four genes in model+SIF group of thoracic aorta were lower than that in model group; there was not significant difference in mRNA level of the four genes between normal control group and normal+SIF group. Immunohistochemistry results showed that expression of positive staining and range of MCPIP, MCP-1,and NF-κB in model group were higher than in normal control group（P<0.01）, the expression of positive staining and range of the three protein in model+SIF group were lower than in model group（P<0.05）. Between the normal control group and the normal+SIF group, the expression of positive staining and range of the three protein were no significant difference. Conclusion: SIF can obviously decrease the expression of genes related to proinflammatory of As , and inhibit the atherosclerosis formation in rats.

Received: 03 January 2014

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2014/V24/I02/99

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