HPLC法同时测定金针菇中麦角甾醇和22,23二氢麦角甾醇的含量

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Abstract Objective: To develop an efficient high performance liquid chromatography (HPLC) method for the simultaneous determination of ergosterol and 22,23dihydroergosterol in Flammulina velutipes. Methods: Chromatographic separation was achieved on an Inertsil ODS-SP (250 mm×4.6 mm, 5 μm) analytical column using a mobile phase of 100% methanol at 1.0 mL·min-1. The sterol content was detected at 282 nm. The temperature of column was 25 ℃. Results: The established linearity range for ergosterol and 22,23-dihydroergosterol was 0.025-1.5 μg (r=0.999 9). The average recoveries (n=6) were 104.6% with RSD 0.89%,103.5% with RSD 3.34%,respectively. Conclusion: The established HPLC method was simple,rapid and reliable for the separation and simultaneous determination of ergosterol and 22,23-dihydroergosterol in Flammulina velutipes.

Received: 01 January 2014

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2014/V24/I02/126

\[1\]郭美英. 中国金针菇生产\[M\]. 北京：中国农业出版社， 2000： 18.
\[2\]Smiderle FR, Carbonero ER, Sassaki GL, et al. Characterization of a heterogalactan: some nutritional values of the edible mushroom Flammulina velutipes\[J\]. Food Chem, 2008, 108(1): 329-333.
\[3\]Shin KS, Yu KW, Lee HK, et al. Production of anti-complementary exopolysaccharides from submerged culture of Flammulina velutipes\[J\]. Food Technol Biotechnol, 2007, 45(3): 319-326.
\[4\]Yi CX, Fu M, Cao X, et al. Enhanced oral bioavailability and tissue distribution of a new potential anticancer agent, Flammulina velutipes sterols, through liposomal encapsulation\[J\]. J Agric Food Chem, 2013, 61(25): 5961-5971.
\[5\]Takaku T, Kimura Y, Okuda H. Isolation of an antitumor compound from Agaricus blazei Murill and its mechanism of action\[J\]. J Nutr, 2001, 131(5): 1409-1413.
\[6\]Zhao YY, Xie RM, Chao X, et al. Bioactivity-directed isolation, identification of diuretic compounds from Polyporus umbellatus\[J\]. J Ethnopharm, 2009, 126(1): 184-187.
\[7\]张萍，肖新月，黄玮，等. RP-HPLC-UV法测定5种发酵虫草制剂中麦角甾醇的含量\[J\]. 药物分析杂志，2011，31(2)：258-260.
\[8\]赵英永，赵晔，林瑞超，等. RP-HPLC法测定猪苓中麦角甾醇的含量\[J\]. 药物分析杂志，2009，29(6): 898-900.
\[9\]Phillips KM, Horst RL, Koszewski NJ, et al. Vitamin D4 in mushrooms\[J\]. PLoS ONE, 2012, 7(8): e40702.
\[10\]Arnezeder Ch, Koliander W, Hampel WA. Rapid determination of ergosterol in yeast cells\[J\]. Analytica Chimica Acta, 1989, 225: 129-136.
\[11\]Larsen T, Axelsen J, Ravn HW. Simplified and rapid method for extraction of ergosterol from natural samples and detection with quantitative and semi-quantitative methods using thinlayer chromatography\[J\]. J Chromatogr A, 2004, 1026(1/2): 301-304.
\[12\]Phillips KM, Ruggio DM, Horst RL, et al. Vitamin D and sterol composition of 10 types of mushrooms from retail suppliers in the United States\[J\]. J Agric Food Chem, 2011, 59(14): 7841-7853.