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High performance liquid chromatography determination method for three monomers of ginkgolic acid in mouse plasma |
YANG Xiao-ming1, SHEN Jie-hong2, LI Yue-ying3, LIN Jian-wei1, HUANG Bing-zhong4 |
(1.School of Food and Biological Engineering, 2.Jingjiang College, 3.School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang Jiangsu 212013; 4.Internal Medicine, the People′s Hospital of Dantu, Zhenjiang Jiangsu 212028, China) |
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Abstract Objective: To develop a high performance liquid chromatography (HPLC) method for determining three monomers of ginkgolic acids, GA 13∶0, GA 15∶1 and GA 17∶1, in mouse plasma. Methods: Plasma samples were treated with acetonitrile for protein precipitation. The analytical column was SinoChrom ODSAP C18(250 mm×4.6 mm, 5 μm).The mobile phase consisted of a mixture of methanol3% acetic acid (92∶8) at a flow rate of 1.0 mL· min-1. The detection wavelength was at 310 nm. Results: Standard curves of GA 13∶0, GA 15∶1 and GA 17∶1 were linear in the range of 0.135-20 μg·mL-1, 0.129-20 μg·mL-1, and 0.211-22 μg·mL-1, respectively (both r>0.9990). The lower limit of quantification were 0.135, 0.129 and 0.211 μg·mL-1, respectively. The extraction recoveries>70.2% (n=5) and the accuracies were 95.2%-101.5%. The intra-day and inter-day precisions were lower than 6.2% and 9.5 % (n=5), respectively. Conclusion: The HPLC method was successfully built for determining GA 13∶0, GA 15∶1 and GA 17∶1, which was simple and sensitive, accurate with reproducible, and suitable for clinical pharmacokinetic study. [Key words]ginkgolic acid monomer; high performance liquid chromatography; mouse
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