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Aqueous two-phase system coupled with heat reflux for the extraction of tetrandrine from Stephania tetrandra S. Moore |
| LUO Kai-wen, QI Xue-yong, GE Yan-ru, JIANG Xue-yin, ZHU Jing-jing, FU Hai-zhen |
| (School of Pharmacy, Jiangsu University, Zhenjiang Jiangsu 212013,China) |
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Abstract Objective: To improve the method of extraction and separation of tetrandrine from Stephania tetrandra S. Moore in order to reduce environment pollution and cut down the cost of production. Methods: The ethanol/(NH4)2SO4 as a two aqueous phase system for the extraction and separation of tetrandrine was studied.The effect of operating parameters, including the concentration of ethanol and (NH4)2SO4,pH and temperature on extraction was studied in depth. Results: The extraction rate of tetrandrine in the aqueous two phase system could reach 84.50%. The ratio of solid:solvent was 1∶8, the time of heat reflux were 2 h, the salinity of (NH4)2SO4 was 35%, the density of ethanol was 20% (w, %), the pH level was 9, accordingly. The purity and yield of tetrandrine crude were 20.30% and 19.80 mg/g accordingly after recycling ethanol.Conclusion: The method of aqueous two phases could be applied to extraction and separation of tetrandrine in industry due to conveniently of procedure, highly of extraction rate and repeatability.
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Received: 23 April 2013
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2013, Vol. 23 
