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| RNA interference in pseudogene Cripto-3 on proliferation and invasion of human colon cancer cell |
| ZHU Ling, JIANG Peng-cheng, MAN Chang-feng, PENG Hui-yong, XU Juan, FAN Yu |
| (Institute of Oncology, The Affiliated People′s Hospital of Jiangsu University, Zhenjiang Jiangsu 212002, China ) |
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Abstract [Abstract] Objective: To study the effects of RNA interference-mediated silencing of pseudogene Cripto-3(Cr-3) on proliferation and invasion of colon cancer cells. Methods: The cells were divided into five groups, blank control group (Con-A group), empty control group (Con-B group), siRNA transfection group (5 nmol/L group, 10 nmol/L group and 20 nmol/L group). After human colon cancer cell line SW480 were transfected by Cr-3 small interfering RNA (siRNA), the levels of Cr-3 were determined by quantitative real-time PCR. The cell growth and invasion ability was examined by clone formation in soft agar, and Transwell, respectively. Results: Compared with Con-A group and Con-B group, siRNA transfection group (5 nmol/L group, 10 nmol/L group and 20 nmol/L group) had lower levels of Cr-3 mRNA expression with lower invasion ability of colon cancer cells and in a dosedependent manner(both P＜0.05). Conclusion：Cr-3 might play an important role in the development of human colon cancer, and down-regulation of Cr-3 by its siRNA could inhibit the invasion of human colon cancer cells.
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Received: 28 March 2013
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2013, Vol. 23 
