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| Establishment of a mouse model of human tumor based on principle of oral tolerance |
| LIU Xia1, CHENG Jing1, FAN Xing-wen1, ZHOU Xiao-bing2, ZHOU Dan1,
WU Wei-jiang1, HUA Ye3, SHAO Qi-xiang1 |
| 1.School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang Jiangsu 212013; 2.Department of General Surgery, the Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001; 3.Department of Oncology, the Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001, China |
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Abstract Objective: To establish a mouse model of human tumorspecific immune tolerance based on principle of oral tolerance. Methods: Ninety mice were randomly divided into three groups: blank control group, irrelevant tumor control group and experimental tumor model group, thirty in each group. The mice of three groups were received intragastric administration of normal saline, human ovarian cancer cell line 3AO and human hepatoma cell line HepG2 cells for 7 days, respectively. Then, all mice received subcutaneous injection of HepG2 cells in the back skin. The time course of the tumor incidence were recorded and the size of tumor were determined by slide caliper. The histological examination of sections of tumor tissues were evaluated by optical microscope. Results: HepG2 cells in mice from experimental tumor model group survived and developed into tumor after subcutaneous injection of HepG2 cells. The tumor incidence was 90％ (27/30). There was no tumor formation in the mice of blank control group and irrelevant tumor control group. Obviously pathologic changes were found in tumor tissue sections with HE staining, while some debris of death tumor cells were found in mice from control group and irrelevant tumor control group. Conclusion: The mouse model of human tumor was established successfully by intragastric administration of HepG2 cells through induction of specific immune tolerance.
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Received: 19 March 2013
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2013, Vol. 23 
