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Expressions of Notch receptors and target gene in cultured fibroblast-like synoviocytes from osteoarthritis |
| WANG Hui1, HUA Shen-hao1, WANG Wen-hong2, WANG Xu-hui1,LIU Qiang1, CHEN Yan1,YOU Hai-yan1, JIAO Zhi-jun1 |
| (1.Department of Laboratory Medicine, the Affiliated Hospital of Jiangsu University，Zhenjiang Jiangsu 212001；2.School of Medical Science and Laboratory Medicine，Jiangsu University，Zhenjiang Jiangsu 212013, China) |
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Abstract Objective：To investigate the expression of Notch signaling molecules in fibroblast-like synoviocytes(FLS) from patients with osteoarthritis(OA). Methods: OA FLSs were isolated from synovial tissues and treated with LPS or LPS + DAPT between the passages of 3 and 5. RT-PCR was used to detect the expression of mRNA of the Notch receptors and Notch target gene Hes-1. In addition, the protein level of Hes-1 was detected by Western blot. Results: Compared with normal FLSs, the mRNA expression of Notch receptors 1, 2, 3 and Hes-1 was markedly increased in OA FLSs(P<0.05). LPS treatment significantly up-regulated the expression of Notch receptors1, 2, 3(Notch 4 level was low) and Hes-1. However, DAPT, a Notch signaling inhibitor, could down-regulated the protein level of Hes-1. Conclusion: Notch signaling was found to be activated in OA FLS, which may be involved in the pathophysiology of the inflammatory in OA joints.
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Received: 21 February 2013
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2013, Vol. 23 
