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| Epstein-Barr virus-encoded BARF1 gene upregulates bcl-2 expression through activating NF-κB signaling pathway in human nasopharyngeal carcinoma cells |
| XU Mei-qin1, LI You-qiong2, ZHANG Xue-yi1, CHU Fu-ying1, ZHANG Yu-qiong1, CHEN Qiao-lin1, CHEN Jing1, ZHOU Tian-ji1 |
| (1.School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang Jiangsu 212013; 2.Department of Laboratory Medicine, the People′s Hospital of Guangxi Zhuang Autonomous Region，Nanning Guangxi 530022, China) |
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Abstract Objective: To investigate the effect of Epstein-Barr virus-encoded BARF1 gene on the nuclear translocation of NF-κB and bcl-2 expression in human nasopharyngeal carcinoma cells. Methods: Fluorescence antibody staining and Western blot were separately performed to analyze the nuclear translocation of NFκB and bcl-2 expression in BARF1-stably-expressing human nasopharyngeal carcinoma cell line CNE1 (CEN1-BARF1) under the control of pSG5 vector transfected one (CEN1-SG). Results: Compared with the control, the nuclear translocation of NF-κB was significantly induced, and bcl-2 expression was significantly increased in CEN1-BARF1. And both were blocked by the specific inhibitor of NF-κB Bay11-7082. Conclusion: BARF1 gene upregulated bcl-2 expression through activating NF-κB signaling pathway in human nasopharyngeal carcinoma cells.
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Received: 28 February 2013
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2013, Vol. 23 
