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| Effects of alpha-lipoic acid on TGF-β1/Smads signal transduction pathway in cardiac fibroblast under high glucose condition |
| WANG Guo-xian, LIU Shan-shan, LI Fei, LI Zhao-gang, LI Rui-fang |
| (Department of Pharmacology， Liaoning Medical College, Jinzhou Liaoning 121001， China) |
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Abstract Objective: To investigate the effects of alpha-lipoic acid (α-LA) on TGF-β1/Smads signal transduction pathway in cardiac fibroblasts(CFb) under high glucose condition. Methods: The primarily cultured neonatal rat CFb were divided into five groups in accordance with different nutrient solutions: NG group(Normal Glucose, 5.5 mmol/L glucose)，HG group(High Glucose ,25.5 mmol/L glucose)，and medication groups: HG+100 μmol/L alpha-lipoic group，HG+200 μmol/L alphalipoic group，HG+300 μmol/L alphalipoic group. CFb prolifertion was measured by thiazolyl blue(MTT) assay. The collagen Ⅰ contents were detected by enzyme linked immunosorbent assay(ELISA), and the protein expression levels of TGF-β1, p-Smad2/3, Smad7 were detected by Western blot. Results: Compared with those of the NG group, in HG group, the CFb proliferation was stimulated, MTT-OD was concomitantly increased（P<0.01），the secretion of collagen Ⅰ was increased（P<0.01)，the protein expression levels of TGF-β1,p-Smad2/3 were also significantly raised, the protein expression levels of Smad7 was reduced; however, after being processed by alpha-lipoic(100, 200, 300 μmol/L)，its CFb proliferation, secretion of collagen Ⅰ and the protein expression level of TGF-β1, p-Smad2/3 were all significantly reduced. Compared with those in the HG group，the protein expression levels of Smad7 was up-regulated. Conclusion: Alpha-lipoic suppressed the proliferation of CFs induced by high glucose, which was possibly related with the suppression of TGF-β1/Smads signal transduction pathway.
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Received: 07 March 2013
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2013, Vol. 23 
