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| Optimization on the cultivation method in vitro of cerebral astrocytus from mice |
| LIU Xiao-mei1, ZHAO Dan2, PANG Rong-rong2, SHAN Kai2, LI Yan2, WANG Ying-wei2 |
| 1.Department of Microbiology and Immunology， Xuzhou Medical College， Xuzhou Jiangsu 221002； 2.Department of Microbiology and Immunology， Nanjing Medical University， Nanjing Jiangsu 210029， China |
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Abstract Objective: To optimize the cultivation method of cerebral astrocytes from mice in vitro, in which develop an experimental basis for the functional study of astrocytus. Methods: Cerebral cortex was obtained from 0 to 1d C57BL/6 mice and cell suspension was prepared by digestion with trypsin digestion and mechanical dissociation. After differential attachments, primary cells were cultivated for 24 h, so the fresh culture medium instead of the old was added softly. After then, the cell flask was washed shakely before the old culture medium was replaced with the fresh medium every two or three days. After three passages, astrocytus in the culture medium were stained with anti-glial fibrillary acidic protein (GFAP) antibody using immunocytochemical or immunofluorescence staining. Results: After primary cell culture and subculture, the purified astrocytes had the typical shapes with a purity beyond 95%. Conclusion: A reproducible and simple cultivation method in vitro of astrocytus was established, in which high purity astrocytes were obtained.
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Received: 29 September 2012
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2012, Vol. 22 
