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| Expressions of CD123 in childhood Blineage acute lymphoblastic leukemia and the application of CD123 in minimal residual disease detection |
| JI Xue-Qiang, Ji Zheng-Hua, Shao Hui-Jiang, et al |
| Department of Clinical Laboratory and Hematology Oncology Laboratory, Soochow University Children′s Hospital, Soochow Jiangsu 215003, China |
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Abstract Objective: Detecting the expression of CD123 in childhood Blineage acute lymphoblastic leukemia (B-ALL) and discussing the application and significance of CD123 in minimal residual disease (MRD) detection. Methods： With the normal children bone marrow lymphocytes as control, multiparameter flow cytometry was used to study the bone marrow lymphocytes immunophenotype and the expression of CD123 in 91 cases of children with B-ALL, 78 cases of which were underwent bone marrow cells chromosome cultivation and cytogenetic analysis. 65 cases of B-ALL children with CD123 positive expression were screened and monitored by CD10/CD123/CD34/CD19 in MRD detection. Results： The expression of CD123 in normal children bone marrow lymphocytes was negative, while 65 cases of 91 in B-ALL children was positive(positive rate 71.43%), and the expression level of CD123 was negatively correlated with the leukemic cells maturity. When children had a high expression of CD34, the expression of CD123 was also in a high level. The results of cytogenetic analysis showed that hyperdiploid B-ALL children had a higher level of CD123 expression, when comparing with the nonhyperdiploid cases. Fortyseven cases of B-ALL children (51.65%) had a high level of CD123 expression, and CD123 could be used as an effective marker for MRD monitoring. Conclusion： Most of B-ALL children had an expression of CD123, which was negatively correlated with the leukemic cells maturity. CD123 can be used as a maker for MRD monitoring in childhood B-ALL.
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. [J]. Journal of Jiangsu University(Medicine Edition), 2019, 29(01): 82-83. |
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2012, Vol. 22 
