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| Expression of TLR2，TLR4 on doxorubicininjured cardiac cells in newborn rats |
| SHU Jin, LIU Xin, WANG Hai-bo,et al |
| Department of Pediatrics, the Affiliated People′s Hospital of Jiangsu University, Zhenjiang Jiangsu 212002, China |
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Abstract Objective: To study the expression of Tolllike receptor 2（TLR2）,Tolllike receptor 4（TLR4） on doxorubicininjured cardiac cells in newborn rats，and to discuss the possible mechanism on doxorubicininjured cardiac cells. Methods: Doxorubicin was administered to establish the injury model of primary cultured cardiomyocytes in newborn rats. Primary cultured cardiomyocytes were randomly divided into two groups: control group：myocardial cell suspension continued cultivate with serumfree medium and no drugs put into; DOX group: with doxorubicin 1 mg/L in cultured fluid.The expression of TLR2 and TLR4 protein in cardiac cells was checked with immunohistochemical after they were operated for 6 hours. The semiquantitative analysis of TLR2 and TLR4 protein was checked with Western blot after they were operated for 2 hours, 6 hours respectively. Results: The expression of TLR2 and TLR4 on the cardiac cells in DOX group are markedly increased when compared with control group(P<0.01). Conclusion: TLR2 and TLR4 may mediate and take part in the innate immune response of doxorubicininjured cardiac cells as a pattern recognition,and this was one of the cardiac cells injuring mechanism.
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Received: 18 July 2012
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| [1] |
. [J]. Journal of Jiangsu University(Medicine Edition), 2019, 29(02): 173-175. |
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2012, Vol. 22 
