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| Umbilical cord blood MSCs transplantation myocardial repair of acute myocardial infarction in rats |
| GONG Min， LI Tao， LEI Jing-wei， XIN Wei-chuan |
| Department of Cardiology, Xi′an Central Hospital， Xi′an Shanxi 710061, China |
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Abstract Objective: To observe the induction of myocardial ischemia microenvironment in vivo, and how human umbilical cord blood mesenchymal stem cells(UCBMSCs) differentiate directly to myocardiallike cells, and their effect on the heart function and revascularization in acute myocardial infarction(AMI) rat and to explore their possible mechanism. Methods: Collected health maternal cord blood cells; density gradient centrifugation and differential speed adherent was used to isolate MSCs. Thirty healthy adult SD rats was preparated to establish rat AMI model. They were divided into 2 groups: transplantation group was peripheral injected of transplanted GFPlabeled UCBMSCs, and control group injected of normal saline. Four weeks later, echocardiography figure detection and hemodynamic check was used, and heart tissue frozen section was harvested to be tested by immunofluorescence staining cbMSCs myocardial specificity protein cTnT and Connexin43 expression; and antiⅧ immunohistochemical staining, myocardial capillary density(MCD)as well. Results: Compared with the control group, 4 weeks after transplantation UCBMSCs expression of cardiacspecific proteins cTnT Connexin43, the control group had no cardiacspecific protein expression; transplantation group LVEDD, LVESD significantly reduced LVEF, LVFS increased significantly,and the hemodynamic indicators improved significantly; immunohistochemical staining showed that the transplantation group infarct border zone MCD increased significantly compared with the control group, the transplantation group(4.16± 0.2)/HP(×400), and the control group(2.29±0.3)/HP; difference between the two groups was significant(P<0.01) Conclusion：UCBMSCs transplantation was survival after rats AMI in their infarct parts and cardiomyocytelike cell differentiated; UCBMSCs transplantation significantly improved AMI cardiac function and ventricular remodeling, and promoted angiogenesis.
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2012, Vol. 22 
