Inhibition of liver lipid deposition by exosomes derived from hepatocytes in mice with nonalcoholic fatty liver
WU Yanshuang1, WANG Yanjin1, YANG Fuji1, YAN Yongmin1,2
(1. School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013； 2. Department of Liver Disease, Third Hospital of Zhenjiang, Zhenjiang Jiangsu 212005, China)
Abstract：［Abstract］Objective： To investigate the effect of exosomes derived from hepatocytes(LO2-Ex) on liver lipid deposition in mice with nonalcoholic fatty liver induced by highfat diet. Methods： Normal human hepatocyte line LO2 cells were cultured and LO2-Ex was extracted by ultracentrifugation. The particle size and concentration of exosomes were measured by nanosight nanoparticle analysis system, the morphology of exosomes was observed by transmission electron microscope, and the markers of exosomes were detected by Western blotting. C57BL/6 male mice were fed with highfat diet for 10 weeks to establish the mice model of nonalcoholic fatty liver. From the 11th week, LO2-Ex was injected into the caudal vein continuously for 4 weeks, and the liver tissue was collected after 4 weeks, the liver tissue structure was observed by HE staining, the liver index(liver mass / net weight) was calculated, the lipid deposition in the liver was detected by oil red O staining, and the collagen deposition in the liver was observed by Sirius red staining. Results： LO2-Ex, a particle size of about 120 nm, has a lipid bilayer structure characteristic of exosomes. It expresses exosome specific proteins CD9 and TSG101, but does not express Calnexin. Compared with the model group, the liver surface of mice in LO2-Ex group were ruddy, delicate and shiny. LO2Ex can reduce the weight of mice and improve the liver index of mice. HE staining, oil red O staining and Sirius red staining showed that LO2-Ex reduced vacuolar degeneration, fat deposition and collagen deposition in liver tissues. Conclusion：LO2-Ex can inhibit lipid deposition in liver tissues and inhibit the progression of nonalcoholic fatty liver in mice.
Key words：
收稿日期: 2022-02-24
基金资助:［基金项目］江苏省科技项目(BE2021690)
通讯作者:
严永敏(通讯作者)，教授，博士生导师，E-mail：yym@ujs.edu.cn
作者简介: 武艳霜(1996—)，女，硕士研究生；
引用本文:
武艳霜，王岩金，杨馥吉，等. 肝细胞外泌体抑制小鼠非酒精性脂肪肝脂质沉积[J]. 江苏大学学报:医学版, 2022, 22(04): 332-337.
WU Yanshuang1, WANG Yanjin1, YANG Fuji1, YAN Yongmin1,2. Inhibition of liver lipid deposition by exosomes derived from hepatocytes in mice with nonalcoholic fatty liver. Journal of Jiangsu University(Medicine Edition), 2022, 22(04): 332-337.
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