Abstract：Objective: To investigate the optimal proportion of silk fibroin/chitosan 3D porous scaffolds for bone tissue engineering. Methods: 3D silk fibroin/chitosan porous scaffolds were constructed by mixing the silk fibroin solution with chitosan solution in freezing-drying.The surface morphology and porosity of the scaffolds were studied by Scanning Eelectronic Microsope(SEM).MG-63 cells were co-cultured with silk silk fibroin/chitosan scaffolds in order to evaluate the compatibility of the materials. Cell adhesion rate was calculated by cell count and cell proliferation was detected by MTT. The morphology of the cells in the scaffold was observed by SEM.The osteogenic property of silk fibroin/chitosan 3D scaffolds was observed by detecting alkaline phosphatase activity. Results: SEM results showed that 50% silk fibroin, 40% silk fibroin scaffolds have regular pore structure. SEM also showed that MG-63 cells in 50%silk fibroin and 40% silk fibroin scaffolds grew well, and the cells of 50% silk fibroin survived more than the number of 40% silk fibroin.The ALP activity of MG-63 cells in 50% silk fibroin scaffold was higher than that in 40% silk fibroin group. Conclusion: In each group, 50% silk fibroin scaffolds had the best surface morphology. The 50%silk fibroin was most suitable for MG-63 cells adhesion and proliferation of osteoblasts.
［Key words］bone tissue engineer; silk fibroin; chitosan; 3D scaffold; MG63 cell
2019, Vol. 29 
