高良姜素的分离纯化及降尿酸作用

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摘要目的: 建立硅胶柱层析法分离纯化高良姜素，探讨高良姜素对急性高尿酸血症小鼠尿酸的影响及其机制。方法：采用硅胶柱层析法从高良姜中分离纯化高良姜素，用薄层色谱法(TLC)和高效液相色谱法(HPLC)对高良姜素进行定性、定量分析。建立次黄嘌呤和氧嗪酸钾诱导的小鼠急性高尿酸血症模型，将70只昆明小鼠分为正常对照组、阳性对照组（别嘌醇溶液灌胃）、溶剂对照组（羧甲基纤维素钠灌胃）、模型组以及高良姜素高中低3个剂量组，检测各组小鼠血清尿酸值及肝脏黄嘌呤氧化酶(XOD)活性。结果：成功分离纯化高纯度的高良姜素(94.14%)；成功建立小鼠高尿酸模型；造模1 h后，与模型组尿酸\[(230.18±32.15)μmol·L-1\]相比，阳性对照组\[(51.80±19.72)μmol·L-1\]和正常对照组\[(62.76±27.73)μmol·L1\]尿酸水平明显下降(P<0.01)；分组给药2 h后，与模型组相比，高良姜素高剂量组\[(98.76±24.25)μmol·L-1\]与中剂量组\[(104.47±28.58)μmol·L-1\]尿酸水平明显降低(P<0.05)；与模型组［(119.29 ±29.34)μmol·L-1］相比，高剂量组XOD活性\[(93.32±29.57)μmol·L-1\]明显降低(P<0.05)。结论：分离纯化获得的高纯度高良姜素，可降低高尿酸血症小鼠的血尿酸浓度，其作用机制可能是通过抑制黄嘌呤氧化酶活性减少尿酸生成。

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关键词 ：高良姜素, 高尿酸血症, 黄嘌呤氧化酶

Abstract：bjective: To investigate the effect of galangin on uric acid in acute hyperuricemia in mice after galangin was separated and purified from Rhizoma Alpiniae Officinarum. Methods: Galangin was extracted and purified from Rhizoma Alpiniae Officinarum using silica gel column chromatography alongside high performance liquid chromatography(HPLC) and thinlayer chromatography(TLC) were applied for qualitative and quantitative analysis of galangin. The acute hyperuricemia model of mouse was induced by hypoxanthine and potassium oxonate, all 70 Kunming mice were equally divided into seven groups: normal control group, positive control group (received intragastric administration of allopurinol), solvent control group (received intragastric administration of carboxymethyl celluose sodium), model group and galangin with high, moderate and low dose groups. Afterwards the level of uric acid and xanthine oxidase (XOD) activity in liver were detected. Results: The purity of the isolated galangin was 94.14%. The uric acid level of the normal group\[(62.76±27.73)μmol·L-1\] and positive control group \[(51.80±19.72)μmol·L-1\] was significantly lower than that of the model group \[(230.18±32.15)μmol·L-1 , P<0.01)\]. In addition, the uric acid level in mice of high and moderate dose galangin groups were lower than that of model group (P<0.05). Compared with the model group, the XOD in moderate dose galangin group was decreased significantly(P<0.05). Conclusion: Galangin was successfully isolated and purified from Rhizoma Alpiniae Officinarum. And it could reduce serum uric acid level in hyperuricemic mice, which might be attributed via the inhibition of xanthine oxidase activity.

收稿日期: 2017-04-17

基金资助:

国家自然科学基金资助项目(81373371，30973677)

通讯作者: 余江南(通讯作者)，教授，博士生导师，E-mail：yjn@ujs.edu.cn

作者简介: 濮尊琴(1992—)，女，硕士研究生

引用本文:

濮尊琴，王启龙，徐希明，余江南. 高良姜素的分离纯化及降尿酸作用[J]. 江苏大学学报:医学版, 2017, 27(04): 338-343. PU Zun-qin, WANG Qi-long, XU Xi-ming, YU Jiang-nan. Separation, purification of galangin and its effect on reducing uric acid. Journal of Jiangsu University(Medicine Edition), 2017, 27(04): 338-343.

链接本文:

http://zzs.ujs.edu.cn/xbyxb/CN/ 或 http://zzs.ujs.edu.cn/xbyxb/CN/Y2017/V27/I04/338

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