Effect of self-made Tiaoqi Decoction on cisplatin induced renal injury in rats and its mechanism
LI Xiaolan1, WANG Shaojun1, MA Yuefei1, WANG Linlin2
(1. Department of Nephrology, Affiliated Hospital of Institute of Traditional Chinese Medicine, Zhangjiakou Hebei 075000; 2. Department of Nephrology, the First Affiliated Hospital of Hebei North University, Zhangjiakou Hebei 075000, China)
Abstract:Objective: To investigate effect of selfmade Tiaoqi Decoction on cisplatin induced renal injury in rats and its potential mechanism. Methods: Ninety male SpragueDawley rats were randomly divided into normal control group, model group, low, medium and high dose groups of Tiaoqi Decoction, and positive control group (n=15 for each group). Cisplatin (2.5 mg/kg) was injected via the rat′s tail vein to induce kidney injury. The low, medium and high dose groups of Tiaoqi Decoction were administrated to rats at the doses of 2, 4, and 8 mL/kg by gavage. The positive control group was intraperitoneally injected with amifostine at a dose of 1 mg/kg, and the control group and model group rats were administrated with the normal saline (2 mL/kg) by gavage once a day for 60 consecutive days. The contents of serum urea nitrogen and creatinine were determined by colorimetry. The contents of tumor necrosis factorα (TNFα), IL-6 and IL-1β of the kidney tissue, and the contents of TNF-α, IL-17 and matrix metalloproteinase-9 (MMP-9) secreted by spleen lymphocytes were determined by ELISA. The activity of superoxide dismutase (SOD) and the level of malondialdehyde were determined by colorimetric method and colorimetric method, respectively. Pathological changes of rat kidney tissue were examined by HE staining. The protein levels of Tolllike receptor 4 (TLR4) and NFκB in rat kidney tissue were tested by Western blotting. Results: Compared with the model group, urea nitrogen and creatinine levels of the serum, TNF-α, IL-6, IL-1β and MDA levels, TLR4 and NFκB protein levels of the kidney tissue, TNF-α, IL-17, MMP-9 levels of the spleen were significantly decreased in the low, medium and high dose groups of Tiaoqi Decoction (P<0.05), but SOD activity was significantly increased(P<0.05). The pathological changes of the kidney tissue was significantly attenuated. With the dose of Tiaoqi Decoction increasing, dosedependent changes of the indicators above were observed. Conclusion: Selfmade Tiaoqi Decoction could significantly attenuate renal injury induced by cisplatin in rats by inhibiting TLR4/NFκB pathway.
收稿日期: 2020-11-09
基金资助:国家中医药管理局第六批师承项目(人教发[2017]29号)
作者简介: 李小兰(1982—),女,硕士,副主任医师,主要从事肾病、风湿免疫病研究。
引用本文:
李小兰1, 王韶军1, 马跃飞1, 王琳琳2. 自拟调气汤对顺铂诱导的大鼠肾损伤的影响及其机制[J]. 江苏大学学报:医学版, 2021, 31(05): 396-400.
LI Xiaolan1, WANG Shaojun1, MA Yuefei1, WANG Linlin2.
Effect of self-made Tiaoqi Decoction on cisplatin induced renal injury in rats and its mechanism
. Journal of Jiangsu University(Medicine Edition), 2021, 31(05): 396-400.
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