近红外光响应性复合纳米颗粒介导光热消融与自由基生成用于乳腺癌细胞的联合治疗

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摘要目的: 制备新型聚多巴胺复合纳米颗粒，探究其对乳腺癌细胞的联合治疗。方法：以血清白蛋白，2,2-偶氮双［2-（2-咪唑啉-2-基）丙烷］二盐酸盐（AIPH），多巴胺为前驱物，通过一步氧化聚合法制备新型聚多巴胺纳米颗粒（AIPH@PB NPs）；利用马尔文粒度分析仪、X射线光电子能谱和傅里叶红外光谱表征其理化学特性；利用紫外可见光谱表征其产生烷基自由基的能力；利用近红外热成像仪表征其光热转换效能；采用4T1乳腺癌细胞体外验证抑制肿瘤的效果。结果：成功制备具有良好生物相容性的AIPH@PB NPs，其粒径为165 nm。在808 nm近红外激光照射下，AIPH@PB NPs可将光能转化成热能（光热转换效率可高达31%）。同时，局部高温进一步诱导AIPH裂解并产生大量烷基自由基。细胞实验结果显示，AIPH@PB NPs介导光热消融并诱导烷基自由基的产生可协同抑制4T1细胞的生长。结论：成功制备AIPH@PB NPs，其具有较强的光热转换效能与高效的烷基自由基产生能力，可用于乳腺癌细胞的联合治疗。

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	张静静
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	杜凤移
	张礼荣

关键词 ：聚多巴胺纳米颗粒, 烷基自由基, 光热治疗, 乳腺癌细胞

Abstract：Objective: To develop novel polydopamine composite nanoparticles and explore its combined inhibition of breast cancer cells. Methods: Novel polydopamine nanoparticles -AIPH@PB NPs） were prepared by one-step oxidation polymerization using serum albumin（BSA）, 2,2-azobis［2（2imidazoline2yl）-propane- dihydrochloride （AIPH） and dopamine （DA） as precursors. The physical and chemical properties were characterized by Malvern particle size analyzer, Xray photoelectron spectroscopy（XPS） and Fourier infrared spectroscopy. Ultravioletvisible spectroscopy was used to characterize its ability to generate alkyl radicals. The photothermal conversion efficiency was characterized by near infrared thermal imager. The 4T1 breast cancer cells were used to verify the effect of tumor suppression in vitro. Results: AIPH@PB NPs with good biocompatibility and a particle size of 165 nm were successfully prepared. Under the irradiation of 808 nm nearinfrared laser, AIPH@PB NPs could convert light energy into heat energy （photothermal conversion efficiency could be as high as 31%）. Meanwhile, local high temperature further induced the decomposition of AIPH to produce a large number of alkyl radicals. The cell experiment results showed that photothermal ablation and the generation of alkyl radicals mediated by AIPH@PB NPs significantly reduced the activity of 4T1 cells. Conclusion: AIPH@PB NPs was successfully prepared and may， with strong photothermal conversion efficiency and highefficiency alkyl radical generation capacity, be used for combined inhibition of breast cancer cells.

收稿日期: 2021-02-26

基金资助:江苏省自然科学基金资助项目（SBK2020022937，BK20161317）；江苏省青年医学重点人才资助项目（QNRC2016444）；江苏省高校重大自然科学研究项目（19KJA150004）

通讯作者: 杜凤移（通讯作者），副教授，硕士生导师，E-mail：biodfy@ujs.edu.cn

作者简介: 张静静（1994—），女，硕士研究生

引用本文:

张静静，徐丽霞，刘苏婉，刘劲静，杜凤移，张礼荣. 近红外光响应性复合纳米颗粒介导光热消融与自由基生成用于乳腺癌细胞的联合治疗[J]. 江苏大学学报:医学版, 2021, 31(05): 369-373. ZHANG Jingjing, XU Lixia, LIU Suwan, LIU Jinjing, DU Fengyi, ZHANG Lirong.

Near-infrared light-responsive composite nanoparticles mediated photothermal ablation and free radical generation for combined inhibition of breast cancer cells

. Journal of Jiangsu University(Medicine Edition), 2021, 31(05): 369-373.

链接本文:

http://zzs.ujs.edu.cn/xbyxb/CN/ 或 http://zzs.ujs.edu.cn/xbyxb/CN/Y2021/V31/I05/369

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