Abstract：Objective: To explore the effect of microvesicles of Schwannlike cells derived from human neural stem cells on axon growth of neurons. Methods: Dorsal root ganglion(DRG) neurons were obtained from DRG of suckling rats born 4-5 d. DRG neurons were stimulated by microvesicles of Schwannlike cells, and immunofluorescence staining of βⅢ-tubulin was performed to observe the axon growth. After stimulating neuronlike N2a cells with 0, 5, 20, 40 mg/L microvesicles of Schwann-like cells for 19 h, the expression of growth associated protein 43(GAP43) mRNA in N2a cells was detected by realtime PCR. After stimulating neuron-like N2a cells with 0, 20, 40 mg/L microvesicles of schwann-like cells for 48 h, periodic acidSchiff(PAS) staining was performed to detect intracellular glycogen production. Results: The axon length of DRG neurons in the Schwann cell microvesicle group was significantly longer than that in the PBS group(P<0.05). Microvesicles of Schwann-like cells could be internalized by N2a cells, and there was no significant difference in GAP43 mRNA expression between 5 mg/L group and 0 mg/L group(P<0.05). Compared with 0 mg/L group, 20 and 40 mg/L groups showed higher levels of GAP43 mRNA expression, neurite outgrowth rate and PAS staining positive cell rate (all P<0.05).Conclusion: Microvesicles of Schwannlike cells derived from human neural stem cells could promote the growth of murine axons in vitro.
收稿日期: 2019-03-19
基金资助:
国家自然科学基金资助项目(81571221)；江苏省自然科学基金资助项目(BK20151346)
通讯作者:
胡嘉波(通讯作者)，教授，硕士生导师，E-mail：hu@ujs.edu.cn
作者简介: 叶开(1994—)，男，硕士研究生
引用本文:
叶开， 余佳红， 陈天琰， 高建一， 张磊， 胡嘉波. 人神经干细胞源施万样细胞微囊泡促进大鼠神经元轴突生长[J]. 江苏大学学报:医学版, 2019, 29(04): 322-326.
YE Kai, YU Jia-hong, CHEN Tian-yan, GAO Jian-yi, ZHANG Lei, HU Jia-bo. Microvesicles of Schwann-like cells derived from human neural stem cells promote axonal growth of neurons in vitro. Journal of Jiangsu University(Medicine Edition), 2019, 29(04): 322-326.
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2019, Vol. 29 
