Abstract:Objective: To study the therapeutic effect of gemcitabine on experimental autoimmune myocarditis (EAM) model mice. Methods: A total of 15 Balb/c mice were used to construct EAM model and divided into control group, EAM group, EAM+gemcitabine group(n=5 in each group). On the 21st day, the eyeballs blood were harvested and the mice were sacrificed by cervical dislocation. HE staining was performed to observe lymphocyte infiltration in the cardiac tissue, and the inflammation score was analyzed. Flow cytometry was used to detect the proportion of myeloidderived suppressor cell (MDSCs) and Th17 cells in spleen tissues; the concentration of IL17A in serum was detected by ELISA. The IL17A mRNA level in cardiac tissue was detected by qRTPCR. MDSC and Nave CD4+T cells were sorted from spleens of Balb/c mice, and divided into activation group, induction group, coculture group; the Th17 percentage in each group were detected by flow cytometry. Results: Compared with the control group, there was mainly inflammatory cell infiltration and myocardial necrosis in the myocardial tissue of the EAM group. Compared with the EAM group, the infiltration of inflammatory cells and myocardial necrosis were reduced in the EAM+gemcitabine group. Compared with the control group, the myocardial tissue inflammation score of EAM group was significantly increased(P<0.05). Compared with EAM group, the myocardial tissue inflammation score of EAM+Gemcitabine group was significantly lower(P<0.05). Compared with the control group, the ratio of MDSC and Th17 in the spleen of EAM group was significantly increased(P<0.05). Compared with EAM group, EAM+Gemcitabine group showed lower proportion of MDSC and Th17 cells in the spleen(P<0.05). Compared with the control group, the serum IL17A concentration and IL17A mRNA in the EAM group were significantly increased(P<0.05). Compared with EAM group, serum IL17A concentration and IL17A mRNA level in EAM+gemcitabine group were significantly lower(P<0.05). Compared with the induction group, the proportion of IL17A in the coculture group was significantly increased(P<0.05). Conclusion: In the EAM mouse model, gemcitabine may attenuate myocardial inflammation by inhibiting the amount of MDSC in the mouse spleen and the differentiation of Nave CD4+T cells into Th17 cells.
收稿日期: 2018-12-31
基金资助:国家自然科学基金资助项目(81771756)
通讯作者:
许化溪(通讯作者),教授,博士生导师,E-mail:xuhx@ujs. edu.cn
作者简介: 倪道斌(1992—),男,硕士研究生
引用本文:
倪道斌, 吕宏祥, 陈蓉, 田雨, 张士清, 苏兆亮, 许化溪. 吉西他滨对小鼠实验性自身免疫性心肌炎的影响[J]. 江苏大学学报:医学版, 2019, 29(02): 117-122.
NI Dao-bin, LU Hong-xiang, CHEN Rong, TIAN Yu, ZHANG Shi-qing, SU Zhao-liang, XU Hua-xi. Effect of gemcitabine on experimental autoimmune myocarditis in mice. Journal of Jiangsu University(Medicine Edition), 2019, 29(02): 117-122.
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