Abstract：Objective: To investigate the effects of low-zinc diet on long-term seizure threshold, mortality and zinc transporter 3 (ZnT3) in rats with two-hit convulsion model. Methods: A total of 60 postnatal day 27(P27) healthy male Sprague-Dawley rats were randomly divided into 4 groups. In the convulsion group and the convulsion with low-zinc diet group, the seizure persistence model were induced by intraperitoneal injection of lithium chloride in P27 and intraperitoneal injection of pilocarpine in P28. By observing the seizures in rats, the seizures of grade Ⅳ and above are considered successful. The control group and the low-zinc diet group were intraperitoneally injected with physiological saline at the same time.Two days before the convulsions (P27, P28)all rats were fed with the zinc diet (zinc 44 mg/kg). After the convulsions(after P28), the control group and the convulsion group continued to be fed with the zinc diet, while the lowzinc diet group and the convulsive lowzinc diet group were fed a low-zinc diet(zinc 2.7 mg/kg). After 2 weeks, penicillin was given as a second strike, and the time of first seizures(seizure threshold),time of death and mortality were recorded. The observation time was 1 h. At the end of the observation, the cortical tissues of each group were isolated, and the expression of ZnT3 was detected by Western blotting. Results: The success rate of continuous convulsion model in rats was 72.5%(29/40), and the mortality rate was 24.1% (7/29). On the 3rd, 5th, and 7th day of convulsion, the body weight of the convulsion group and the convulsion with low-zinc diet group was significantly lower than that of the control group(P＜0.05).The convulsion on the 9th day was significantly lower than that of the control group（P＜0.05）.The serum zinc concentration in the convulsion group and the convulsion with low-zinc diet group were significantly less than those in the control group（P＜0.05）.The seizure threshold of the low-zinc diet group, the convulsion group and the convulsion low-zinc diet group was significantly lower than that of the control group（P＜0.05）, the convulsion low-zinc diet group was significantly lower than the low-zinc diet group and the convulsion group（P＜0.05）.The survival time of the convulsive lowzinc diet group was significantly lower than the control group and the lowzinc diet group (χ 2=9.994, 6.707, all P＜0.05）. The survival rates of the groups were 80%, 60%, 50%, and 20%, there was no statistical difference(P=0.073). The expression of ZnT3 protein in the lowzinc diet group, the convulsion group and the convulsion low-zinc diet group were significantly lower than that of the control group（P＜0.05）, the convulsion low-zinc diet group was significantly lower than that of the convulsion group and the lowzinc diet group（P＜0.05）. Conclusion: Convulsion can reduce the serum zinc concentration in rats. The zinc deficiency diet can increase the susceptibility of twohit seizure threshold in rats. This synergistic effect is related to the down-regulating the expression of ZnT3 and the zinc dys homeostasis in the brain.
［Key words］zinc; epilepsy; seizure threshold; zinc transporter 3
陈妮娜， 倪宏. 低锌饮食对大鼠二次打击惊厥模型远期惊厥阈和锌转运体3的影响[J]. 江苏大学学报:医学版, 2018, 28(06): 473-.
CHEN Ni-na, NI Hong. Effects of low-zinc diet on long-term seizure threshold and zinc transporter 3 in rats with two-hit convulsion model . Journal of Jiangsu University(Medicine Edition), 2018, 28(06): 473-.
2018, Vol. 28 
