(1. Department of Nephrology, the Affiliated People′s Hospital of Jiangsu University, Zhenjiang Jiangsu 212002; 2. Department of Neurology, the Affiliated People′s Hospital of Jiangsu University, Zhenjiang Jiangsu 212002; 3. Laboratory Animal Center, Jiangsu University, Zhenjiang Jiangsu 212013, China)
Abstract：Objective: To investigate whether geranylgeranylacetone(GGA) could induce diabetic nephrotic rats to express heat shock protein 72(HSP72), and its effect on 24hour urinary protein, and serum creatinine in diabetic nephropathy rats. Methods: The rats were randomly divided into normal control group, diabetic nephropathy group and intervention group. Left nephrectomy and streptozotocin abdominal injection were carried out to establish the rat model of diabetic nephropathy. GGA was given by gavage each day for 12 weeks for the rats of intervention group and the other two groups were given equal volume of the solvent. The expression of HSP72 in the kidney tissue was detected by immunohistochemical staining; and the levels of blood glucose, 24hour urinary protein, and serum creatinine were measured for all groups at the beginning of gavage, 6 weeks and 12 weeks after the gavage, respectively. Results: When GGA had been gavaged for 12 weeks, the expression of HSP72 was detected by immunohistochemical staining in the intervention group, while the other two groups wasn′t in kidney tissues. The PAS staining showed that the basal membrane and the extracellular matrix were normal in control group, but the basal membrane was thickening and the extracellular matrix were increased in the model group.The extracellular matrix deposition in intervention group was less than that of the model group. Compared with the model group, the intervention group had significantly lower levels of 24hour urinary protein and serum creatinine(P<0.05), but there was no statistical difference in the levels of blood glucose. Conclusion: GGA could induce the rat kidney to express HSP72, and significantly reduce the levels of urine protein and serum creatinine in rats with diabetic nephropathy.
收稿日期: 2017-05-02
基金资助:
镇江市社会发展项目(SH2013047)
作者简介: 冯松涛(1983—)，男，河北肥乡人，主治医师，硕士，主要从事糖尿病肾病的研究。
引用本文:
冯松涛1， 陈思远2， 周洁3， 邵维斌1， 夏春英1. 替普瑞酮对糖尿病肾病大鼠尿蛋白及血肌酐浓度的影响[J]. 江苏大学学报:医学版, 2017, 27(5): 412-415.
FENG Song-tao1, CHEN Si-yuan2, ZHOU Jie3, SHAO Wei-bin1, XIA Chun-ying1. Effect of geranylgeranylacetone on the level of urinary protein and serum creatinine of rat with diabetic nephropathy. Journal of Jiangsu University(Medicine Edition), 2017, 27(5): 412-415.
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2017, Vol. 27 
