Hyaluronic acid-functionalized bismuth oxide nanoparticles enhance radiosensitization on hepatoma cells
LOU Jia-ming1, JIN Jie1, GONG Ai-hua2, DU Feng-yi2, WU Chao-yang1
（1.Department of Oncology, the Affiliated People′s Hospital of Jiangsu University, Zhenjiang Jiangsu 212002； 2. School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013，China）
Abstract：Objective: To investigate the radiosensitization enhancements of a newly developed nanopartical modified by hyaluronic acid (HABi2O3) on hepatoma cells SMMC7721. Methods: We used HA and BiCl3·6H2O as precursors to synthesize HABi2O3 by polyol method. HABi2O3 were characterized by transmission electron microscopy(TEM/HRTEM), NanoDLS particle size analyzer and Xray diffraction(XRD).The biocompatibility characterization of HABi2O3 NPs was characterized by CCK8 assay, histological analysis and inductively coupled plasma mass spectrometry(ICPMS). The radiosensitization of HABi2O3 was conducted by CCK8 assay,Livedead staining assay(FDAPI staining) and clonogenic assay, randomly on SMMC7721 cells, divided to four groups (control, HABi2O3, radiation and HABi2O3+radiation). Results: We fabricated the HABi2O3 with a diameter of 45 nm,the amorphous phase structure and these diffraction peaks of HABi2O3 NPs matched well with the characteristic peaks of cubic Bi2O3 (JCPDS 2004 060312). Inhibition rates of cell proliferation of HABi2O3 ranging with different concentrations of HABi2O3(0-400 mg/L) were no statistically significant differences(P＞0.05). HABi2O3 was no obvious toxicity to the mouse susceptible organs (liver, heart, spleen, lung, and kidney) showed by histological assessment and ICPMS. Compared with control, radiation group and HABi2O3 group, there were lower inhibition rates of cell proliferation and survival fraction in HABi2O3+radiation group with statistically significant differences(P＜0.05). Conclusion: We have successfully developed HABi2O3 with favorable solubility in water, excellent biocompatibility, no obvious toxicity and effective radiosensitization enhancement on hepatoma SMMC7721 cells.
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2017, Vol. 27 
