Abstract：Objective: To observe whether CD137 signaling affects the expression of nuclear factor of activated T cells c1(NFATc1) in vascular smooth muscle cells(VSMCs) through CalmodlinCalcineurin(CaMCaN) pathway. Methods: Isolated VSMCs from the mice aortic by explant tissue culture. The experiment was divided into two parts. PartⅠ: the prepared cells were divided into three groups: control group, CD137 activated group, CD137 inhibited group; PartⅡ: control group, CD137 activated group (agonist CD137mAb), siCaM group (siCaM+CD137 activated group), siCaN group (siCaN+CD137 activated group). The levels of CaM, CaN and NFATc1 were evaluated by Western blotting and RTPCR, respectively. Results: (1) The protein and mRNA levels of CaM, CaN and NFATc1 were increased markedly by agitating CD137CD137L signal comparing with control group. When CD137CD137L signaling was restrained by antiCD137 mAb, the protein and mRNA levels of CaM, CaN and NFATc1 were decreased markedly comparing with CD137 activated group. When blocked CaM by CaM siRNA, the protein and mRNA levels of CaM, CaN and NFATc1 were decreased after stimulated by agonist CD137 mAb comparing with CD137 activated group. When blocked CaN, the levels of CaN and NFATc1 were decreased but the level of CaM did not change after stimulated by agonist CD137 mAb comparing with CD137 activated group. Conclusion: CD137CD137L signaling could regulate the expression of NFATc1 through CaMCaN pathway.
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2017, Vol. 27 
