Abstract：［Abstract］Objective： To observe the expressions of connexin40 during the acute lung injury model of C57BL/6 mice and explore the functional correlation between connexin and lung vascular permeability. Methods： C57BL/6 mice were intraperitoneally treated with lipopolysaccharide(LPS,0.01 mg/g) for 24 hours, and then the expressions of connexin40 mRNA and protein levels in lung were measured by realtime PCR and Westernblotting or Fluorescence immunohistology, respectively. To explore correlation between connexin40 and lung vascular permeability,weight gains in isolated perfused lungs at baseline and after bolus infusion of plateletactivating factor(PAF) in the absence (PAF only) or presence(both PAF and gap2740）of gap2740(0.02 mg/mL) were measured by PLUGSYS system, respectively. Results： Realtime PCR and Westernblotting analysis showed that, the expressions of connexin40 mRNA and protein decreased significantly in LPS group compared with control group, which was confirmed by fluorescence imaging. Perfusion of mouse lungs with PAF caused severe edema, but application of gap2740 (connexin40specific inhibitory mimetic peptide) to isolated perfused mouse lungs reduced the extent of PAFinduced edema. Conclusion： The increased lung vascular permeability induced by inflammatory conditions may be amplified by connexin40 despite of its decreased expression.
收稿日期: 2015-12-31
通讯作者:
尹俊（通讯作者），副主任医师，硕士生导师，E-mail:yin912@126.com
作者简介: 翟鹏（1988—），男，硕士研究生；
引用本文:
翟鹏， 尹俊. 缝隙连接蛋白40增加急性肺损伤小鼠肺血管通透性[J]. 江苏大学学报:医学版, 2016, 26(04): 302-306.
ZHAI Peng, YIN Jun. Connexin40 exacerbates lung vascular permeability in mice with acute lung injury. Journal of Jiangsu University(Medicine Edition), 2016, 26(04): 302-306.
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