Abstract:[Abstract]Objective: To investigate the change and potential significance of follicular regulatory T cells (Tfr) percentage in murine model of Lewis lung carcinoma mice. Methods: The mice were injected with the Lewis cells to establish murine model of lung carcinoma. The percentage of Tfr cells and CD4+CXCR5+T cells in abdominal drainage lymph nodes and tumor tissues were determined by flow cytometer.The change at ratio of Tfr/CD4+CXCR5+T cell were analyzed. Results: Compared with the normal control mice (1.97±0.26)%,tumorburdened mice (4.13±0.35)% had a significantly higher percentage of Tfr cells in abdominal drainage lymph nodes(t=4.985, P<0.01). Tfr/CD4+CXCR5+T cell ratio in tumorburdened mice(0.39±0.03) was also obviously increased(t=5.148, P<0.01) compared with the normal control mice(0.21±0.02). The percentage of Tfr cells in tumor tissues had a significantly higher on fourteenth day,the difference was statistically significant compared with seventh days(t=5.617, P<0.01). Conclusion: The increased percentage of Tfr cells in Lewis lung carcinoma mice and the imbalance of Tfr/CD4+CXCR5+T cell ratio indicated that Tfr cells may be involved in the progression of lung carcinoma.
冯玎琦, 汤新逸, 张悦, 马洁, 田洁, 许化溪, 王胜军. 滤泡调节性T细胞在肺癌移植瘤小鼠体内的变化及意义[J]. 江苏大学学报:医学版, 2016, 26(04): 288-291.
FENG Ding-qi, TANG Xin-yi, ZHANG Yue, MA Jie, TIAN Jie, XU Hua-xi, WANG Sheng-jun. Increasement and significance of follicular regulatory T cells in the murine model of Lewis lung carcinoma. Journal of Jiangsu University(Medicine Edition), 2016, 26(04): 288-291.
[1]Linterman MA, Pierson W, Lee SK, et al. Foxp3+ follicular regulatory T cells control the germinal center response\[J\]. Nat Med, 2011, 17(8):975-982.[2]Sage PT, Paterson AM, Lovitch SB, et al. The coinhibitory receptor CTLA4 controls B cell responses by modulating T follicular helper, T follicular regulatory, and T regulatory cells\[J\]. Immunity, 2014, 41(6):1026-1039.[3]Li H, Pauza CD. CD25+ Bcl6(low) T follicular helper cells provide help to maturing B cells in germinal centers of human tonsil\[J\]. Eur J Immunol, 2015, 45(1):298-308.[4]Faghih Z, Erfani N, Haghshenas MR, et al. Immune profiles of CD4+ lymphocyte subsets in breast cancer tumor draining lymph nodes\[J\]. Immunol Lett, 2014, 158(1/2):57-65.[5]Gaulard P, de Leval L. Follicular helper T cells: implications in neoplastic hematopathology\[J\]. Semin Diagn Pathol, 2011, 28(3):202-213.[6]Crotty S. T follicular helper cell differentiation, function, and roles in disease\[J\]. Immunity, 2014, 41(4):529-542.[7]Xiao H,Luo G,Son H,et al. Upregulation of peripheral CD4+CXCR5+ T cells in osteosarcoma\[J\]. Tumour Biol, 2014, 35(6):5273-5279.[8]Xie X, Mu L, Yao X, et al. ATRA alters humoral responses associated with amelioration of EAMG symptoms by balancing Tfh/Tfr helper cell profiles\[J\].Clin Immunol,2013, 148(2):162-176.[9]Blackburn MJ, ZhongMin M, Caccuri F, et al. Regulatory and helper follicular T cells and antibody avidity to simian immunodeficiency virus glycoprotein 120\[J\].J Immunol,2015, 195(7):3227-3236.[10]Miles B, Miller SM, Folkvord JM, et al. Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection\[J\]. Nat Commun, 2015, 6:8608.[11]Sage PT, Sharpe AH. T follicular regulatory cells in the regulation of B cell responses\[J\]. Trends Immunol, 2015, 36(7):410-418.[12]Chavele KM,Merry E,Ehrenstein MR.Cutting edge:circulating plasmablasts induce the differentiation of human T follicular helper cells via IL6 production\[J\]. J Immunol,2015, 194(6):2482-2485.