Effect of ginkgol on the serum levels of IFN-γ，IL-2 and TNF-α in cisplatin chemotherapied Heps hepatocarcinoma xenografts
LIU Ya-li1, YANG Xiao-ming1, LI Yue-ying2, SHI Ya-xiang3, WANG-Xin1
（1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang Jiangsu 212013; 2. School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013; 3. Department of Spleen and Stomach Medicine, Zhenjiang Hospital of Traditional Chinese Medicine, Zhenjiang Jiangsu 212003, China)
Abstract：[Abstract\]Objective: To study tumorinhibitory role of ginkgol in cisplatin chemotherapied Heps hepatocarcinoma xenografts and its effect on serum levels of IFN-γ,IL-2 and TNF-α. Methods: Heps hepatocarcinoma xenograft models were established and randomly divided into the model group (normal saline), cisplatin group(3.75 mg/kg), ginkgol group(40 mg/kg), cisplatin(3.75 mg/kg) plus low dose ginkgol(20 mg/kg) group, cisplatin(3.75 mg/kg) plus high dose ginkgol(40 mg/kg) group. General state was observed in each group. The change of body weight, the inhibition of the tumor growth, as well as indexes of spleen,thymus and liver were calculated. The levels of serum IFNγ,IL2 and TNFα were determined by ELISA. Results: Compared with cisplatin group, cisplatin plus high dose ginkgol group not only showed higher the thymus, spleen and liver index(P<0.05), but also higher the levels of serum IFNγ,IL2 and TNFα(P<0.05). Conclusion: The high dose(40 mg/kg) ginkgol was synergistic to the antitumor effect of cisplatin, which may be attribute to enhancement of host immune function.
刘亚丽1， 杨小明1， 李月英2， 史亚祥3， 王鑫1. 银杏酚对顺铂化疗Heps肝癌小鼠血清干扰素-γ、白介素-2和肿瘤坏死因子-α水平的影响[J]. 江苏大学学报:医学版, 2016, 26(02): 98-101.
LIU Ya-li1, YANG Xiao-ming1, LI Yue-ying2, SHI Ya-xiang3, WANG-Xin1.
Effect of ginkgol on the serum levels of IFN-γ，IL-2 and TNF-α in cisplatin chemotherapied Heps hepatocarcinoma xenografts. Journal of Jiangsu University(Medicine Edition), 2016, 26(02): 98-101.
[1\]Pasini F, Fraccon AP, DE Manzoni G. The role of chemotherapy in metastatic gastric cancer \[J\]. Anticancer Res, 2011, 31(10): 3543-3554.\[2\]倪学文. 银杏酚酸的分离纯化及其生物活性研究\[D\]. 武汉: 华中农业大学, 2004.\[3\]潘红梅, 吴彩娥, 曹福亮, 等. 银杏种仁中银杏酚酸的提取与组分研究\[J\]. 南京林业大学学报: 自然科学版, 2011, 35(6): 29-33.\[4\]Yang XM, Wang YF, Li YY, et al. Thermal stability of ginkgolic acids from Ginkgo biloba and the effects of ginkgol C17:1 on the apoptosis and migration of SMMC7721 cells\[J\]. Fitoterapia, 2014, 98: 66-76.\[5\]Li JT, Zhang JL, He H, et al. Apoptosis in human hepatoma HepG2 cells induced by corn peptides and its antitumor efficacy in H22 tumor bearing mice\[J\]. Food Chem Toxicol, 2013, 51: 297-305.\[6\]孟翠翠, 陈晓品, 罗治彬. 塞来昔布联合替吉奥对胃癌裸鼠移植瘤生长的抑制作用\[J\]. 细胞与分子免疫学杂志, 2013, 29(5): 458-461.\[7\]王云飞, 杨小明, 李月英, 等. 银杏酚对SMMC-7721肝癌细胞和荷H22肝癌小鼠的抗癌作用\[J\]. 江苏大学学报: 医学版, 2013, 23(3): 234-237.
2016, Vol. 26 
