Abstract：[Abstract]Objective: To explore the effect of inhibition of FA/BRCA pathway upstream genes FANCM and USP1 on the sensitivity of lung squamous cell carcinoma SK-MES-1 to cisplatin. Methods: FANCM-siRNA and USP1-siRNA were transfected into SKMES1 cells using RNA interference (RNAi) technique according to manufacture′s instructions. The transfection efficacy was verified by testing the FANCM and USP1 protein expression using Western blotting, which was also used to detect the levels of FANCD2 monoubiquitination. CCK8 technique was conducted to detect the survival of SKMES1 cells treated with cisplatin pretransfection and post-transfection of USP1-siRNA and FANCMsiRNA. Flow cytometry using Annexin V/PI methods was performed to measure cell apoptosis. Immunofluorescence assay was done to determine the expression of FANCD2 nuclear foci. Results: FANCM and USP1 proteins expressions were obviously decreased after transfection of FANCMsiRNA and USP1siRNA compared with before transfection. FANCM gene silence resulted in downregulation of the FANCD2 monoubiquitination and nuclear foci expression. The USP1 silence increased the FANCD2 monoubiquitination levels and nuclear foci expression. Meanwhile, the silencing of the two genes significantly suppressed the cell survival and promoted cell apoptosis induced by cisplatin. The sensitizing effect to cisplatin in silencing USP1 was more obvious than in silencing FANCM. Conclusion: Depletion of FANCM and USP1 gene by RNAi can enhance the sensitivity of lung squamous carcinoma cells to cisplatin through depression of the FA/BRCA pathway DNA repair function.
李玫瑜，李坚，陈康. 抑制肺鳞癌细胞FANCM和USP1基因对顺铂的增敏效应[J]. 江苏大学学报:医学版, 2016, 26(02): 147-153.
LI Mei-yu, LI Jian, CHEN Kang. Effect of sensitization to cisplatin by inhibiting FANCM and USP1 in lung squamous carcinoma cells. Journal of Jiangsu University(Medicine Edition), 2016, 26(02): 147-153.
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2016, Vol. 26 
