Analysis of aminoglycoside modifying enzyme genes and 16S rRNA methylase genes in drugresistant Escherichia coli strains
YUAN Hong-yan1,2, Guo Bin-fang2, YIN Jing-ping1, SUN Qiang1, GAO Jing-jing1, SU Zhao-liang1, WANG Seng-jun1, XU Hua-xi1, MI Zu-huang3
（1. School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang Jiangsu 212013; 2.Blood Transfusion Department, the Second People′s Hospital of Changzhi, Changzhi Shanxi 046000; 3.Wuxi Clone GenTech Institute,Wuxi Jiangsu 214026, China)
Abstract：Objective: To analyze aminoglycoside modifying enzyme genes and 16S rRNA methylase genes in drugresistant Escherichia coli（DRECO）strains, and investigate the aminoglycoside resistance mechanisms. Methods: The expressions of aminoglycoside modifying enzyme genes and 16S rRNA methylase genes in 20 DRECO strains were assayed by polymerase chain reaction(PCR); and positive PCR products were sequenced by PCR direct automated fluorescence method. Results: In the 20 DRECO strains,18 strains expressed aminoglycoside modifying enzyme genes, the positive rate of aac (3)Ⅱ, aac (6′)Ib, ant (3″)I, aadA4/5 and aph (3′)I were 25%, 25%, 5%, 65% and 55%, respectively, only 1 strains expressed rmtB type 16S rRNA methylase gene, the positive rate was 5%, and the rest genes expressions were not detected. Conclusion: The aadA4/5 and aph (3′)I, not 16S rRNA methylase gene, were closely related with the DRECO aminoglycoside resistance.
收稿日期: 2013-04-01
基金资助:
中国博士后基金资助项目（2012M511705）； 江苏省博士后基金资助项目(1102129C)
通讯作者:
苏兆亮（通讯作者），副教授，E-mail：szl30@yeah.net
作者简介: 原鸿雁（1982—），女，山西长治人，硕士研究生；
引用本文:
原鸿雁1,2， 郭兵方2， 尹晶平1， 孙强1， 高晶晶1， 苏兆亮1， 王胜军1， 许化溪1， 糜祖煌3. 耐药大肠埃希菌氨基糖苷类修饰酶和16S rRNA甲基化酶基因的分析[J]. 江苏大学学报:医学版, 2013, 23(5): 419-422,431.
YUAN Hong-yan1,2, Guo Bin-fang2, YIN Jing-ping1, SUN Qiang1, GAO Jing-jing1, . Analysis of aminoglycoside modifying enzyme genes and 16S rRNA methylase genes in drugresistant Escherichia coli strains. Journal of Jiangsu University(Medicine Edition), 2013, 23(5): 419-422,431.
[1]李耘, 吕媛, 薛峰,等. 我国2009至2010年MOHNARIN项目临床分离常见病原菌的耐药监测［J］. 中华检验医学杂志, 2012, 35(1): 67-87.[2]Yamane K, Wachino J, Doi Y, et al. Global spread of multiple aminoglycoside resistance genes［J］. Emerg Infect Dis,2005,11(6): 951-953.[3]Yamane K, Rossi F, Barberino MG, et al. 16S ribosomal RNA methylase RmtD produced by Klebsiella pneumoniae in Brazil［J］. J Antimicrob Chemother, 2008, 61(3): 746-747.[4]Davis MA, Baker KN, Orfe LH, et al. Discovery of a gene conferring multiple-aminoglycoside resistance in Escherichia coli［J］. Antimicrob Agents Chemother, 2010, 54(6): 2666-2669.[5]Wachino J, Shibayama K, Kurokawa H, et al. Novel plasmid-mediated 16S rRNA m1A1408 methyltransferase, NpmA, found in a clinically isolated Escherichia coli strain resistant to structurally diverse aminoglycosides［J］. Antimicrob Agents Chemother, 2007, 51(12): 4401-4409.[6]Zacharczuk K, Piekarska K, Szych J, et al. Plasmid-borne 16S rRNA methylase ArmA in aminoglycoside-resistant Klebsiella pneumoniae in Poland［J］. J Med Microbiol, 2011, 60(Pt 9): 1306-1311.[7]Chatterjee SS, Chen L, Joo HS, et al. Distribution and regulation of the mobile genetic element-encoded phenol-soluble modulin PSM-mec in methicillin-resistant Staphylococcus aureus［J］. PLoS One, 2011, 6(12): e28781.[8]Queck SY, Khan BA, Wang R, et al. Mobile genetic element-encoded cytolysin connects virulence to methicillin resistance in MRSA［J］. PLoS Pathog, 2009, 5(7): e1000533.[9]Miragaia M, de Lencastre H, PerdreauRemington F, et al. Genetic diversity of arginine catabolic mobile element in Staphylococcus epidermidis［J］. PLoS One, 2009, 4(11): e7722.[10]Zhou Y, Yu H, Guo Q, et al. Distribution of 16S rRNA methylases among different species of Gram-negative bacilli with high-level resistance to aminoglycosides［J］. Eur J Clin Microbiol Infect Dis, 2010, 29(11): 1349-1353.[11]Doi Y, AdamsHaduch JM, Paterson DL. Escherichia coli isolate coproducing 16S rRNA methylase and CTX-M-type extended-spectrum beta-lactamase isolated from an outpatient in the United States［J］. Antimicrob Agents Chemother, 2008, 52(3): 1204-1205.[12]糜祖煌, 秦玲. 泛耐药铜绿假单胞菌16S rRNA甲基化酶、氨基糖苷类修饰酶基因研究［J］. 中华医院感染学杂志, 2008, (12): 1656-1658.[13]Wachino J, Yamane K, Shibayama K, et al. Novel plasmid-mediated 16S rRNA methylase, RmtC, found in a proteus mirabilis isolate demonstrating extraordinary high-level resistance against various aminoglycosides［J］. Antimicrob Agents Chemother, 2006, 50(1): 178-184.[14]Doi Y, Arakawa Y. 16S ribosomal RNA methylation: emerging resistance mechanism against aminoglycosides［J］. Clin Infect Dis,2007, 45(1): 88-94.
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