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| Enforced expression and verification of sall4 in HGC27 cell line |
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ZHANG Ling1, GAO Shuo1, ZHANG Bin1, WANG Mei1, XU Xiao1, QIAN Hui1, XU Wen-rong1,2 |
| (1. School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang Jiangsu 212013; 2. Department of Clinical Laboratory, the Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001, China) |
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Abstract Objective: To establish the stable sall4-overexpressing HGC27 cell line, so as to lay a foundation for exploring the role of sall4 in the development of gastric cancer. Methods: pCMV6-entry-sall4 and pCMV6-entry plasmids were transfected into HGC27 cells via Lipofectamine 2000. At 48 h after transfection, cells were then cultured with G418 at a final concentration of 600 mg／L. Finally, stably transfected sublines of HGC27 cells were obtained. The expression of sall4 in stable cell line was determined by RT-PCR and western blotting. Results: The HGC27-sall4 cell line was obtained. The expression level of SALL4 in HGC27-sall4 cells was significantly higher than untransfected HGC27 cells and HGC27-vector cells. Conclusion: We successfully screened out the stable sall4-overexpressing HGC27 cell line.
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Received: 18 March 2013
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2013, Vol. 23 
