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Expression and clinical significance of nuclear transcription factor NF-κB in colon cancer |
PU Yong, CHEN Suo-cheng |
(Department of General Surgery, the People′s Hospital Affiliated to Jiangsu University, Zhenjiang Jiangsu 212002,China) |
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Abstract Objective: To investigate the expression and clinical significance of nuclear factor(NF)-κB in colon cancer. Methods: NF-κB subunit p65 mRNA and protein from 108 cases of colon cancer tissue were detected by RTPCR and Western blot, relationships between them and demographic informations(gender, age, high-fiber diet, alcohol),clinic pathologic factors (tumor location, size, histological type, depth of invasion, differentiation, lymph node metastasis, Dukes points Period) were analyzed. Results: In colon cancer patients, expression of NF-κB subunit p65 mRNA and protein differed with different histological types of tumor, depth of invasion groups, whether lymph node metastasis or not at Dukes stage, in which, NF-κB mRNA and protein expression were significantly higher in the mucinous carcinoma (signet ring cell carcinoma, mucinous adenocarcinoma), invaded serosa, lymph node metastasis and Dukes C+D (P<0.01 or <0.05). However, NF-κB mRNA and protein expression showed no difference with gender, age, highfiber diet, alcohol, and tumor location, size and degree of differentiation (P>0.05). Conclusion: NF-κB expression had something to do with cancer invasion and metastasis; it showed potential value to detect NF-κB subunit p65 mRNA and protein in the diagnosis and treatment of colon cancer.
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