|
|
|
| Relationship between polymorphism sites of adiponection and it′s receptor gene and the susceptibility of colorectal cancer |
| (1.School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang Jiangsu 212013; 2.Department of Clinical Laboratory, Kunshan Hospital Affiliated to Nanjing University of Traditional Chinese Medicine, Kunshan Jiangsu 215300, China) |
|
|
|
|
Abstract Objective: To explore the relationship between polymorphisms in adiponection and adiponection receptor gene and risk of colorectal cancer. Methods: A total of 370 patients with colorectal cancer and 370 healthy individuals were enrolled in the study, DNA was isolated from 3 ml whole blood obtained from each individuals, and the polymerase chain reaction and restriction fragment length polymorphism (PCRRFLP) was applied to detect the genotype of the polymorphisms in adiponection（rs266729, rs822395, rs822396, rs2241766, rs1501299）and adiponection receptor gene(rs12733285, rs1342387). The frequencies distribution of all genotypes in cancer and control group were compared. Results: There was significant difference of genotype distributions for the polymorphism site(rs12733285) in adiponection receptor gene in the colorectal cancer group（CC 91.89%, CT 8.11%, TT 0.0%）and control group（CC 86.49%, CT 13.51%, TT 0.0%）（χ2=5.61, P=0.018）; ② There was significant difference distribution of rs1342387 polymorphism in adiponection receptor gene genotypes in cancer group（GG 48.65%, AG 38.92%, AA 12.43%）and control group（GG 37.84%, AG 46.49%, AA 15.68%）（χ2=8.87, P=0.012）. ③ The CT genotype of rs12733285 was associated with decreased CRC risk(CT versus CC:adjusted OR=0.56, 95% CI=0.35-0.91), and the AG and AA genotypes of rs1342387 were associated with decreased CRC risk(AG versus GG: adjusted OR=0.66, 95% CI＝0.48-0.90; AA versus GG: adjusted OR=0.62, 95% CI＝0.39-0.98; AG/AA versus GG: adjusted OR=0.65, 95% CI＝0.48-0.87）. ④No significant difference of genotype frequencies distribution was observed for the polymorphism sites in adiponection gene between cancer group and control group. Conclusion: Polymorphism sites in adiponection receptor gene(rs12733285、rs1342387) were associated with colorectal cancer risk.
|
|
|
|
|
|
|
|
|
2012, Vol. 22 
