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| Mechanism of miR let-7b inhibiting breast cancer MCF-7 cells migration |
| （1.Graduate School, 2.Department of CT, the Affiliated Hospital, 3.Department of Tumor Chemoradiation, the Affiliated Hospital, 4.Department of Thoracic Surgery, the Affiliated Hospital of Chengde Medical College, Chengde Hebei 067000, China） |
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Abstract Objective: To observe the effect of miR let-7b on inhibiting breast cancer MCF-7 cells migration and to investigate it′s mechanism. Methods: miR let-7b were composed and transfected into breast cancer MCF-7 cells. MiR-control was taken as a reference. The migration of the cells was tested by scratch test and the IL-6 level of MCF-7 cells before and after transfected was tested by PCR and west-blot. Then the combination of miR let-7b and IL-6 was verification by Bioinformatics software and Dual luciferase. Results: Compared with the control group,miR let-7b made the migrating ability of MCF-7 cells reduced,while the anti-miR let-7b strengthen it.Those breast cancer MCF-7 cells which were transfected by miR let-7b had a lower level of IL-6,while those transfected by anti-miR let-7b had a higher level of IL-6.It was verificated by Bioinformatics software and Dual luciferase that miR let-7b was combined with IL-6. Conclusion: miR let-7b inhibited the expression of IL-6 of breast cancer MCF-7 cells at the genetic level and then inhibited the migration.
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2012, Vol. 22 
