阿尔茨海默病病变相关脑区Pgc-1alpha敲除小鼠模型的构建

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Abstract Objective： To constuct peroxisome proliferator-activated receptor γ coactivator-1 alpha (Pgc-1alpha) knockout mouse model in Alzheimer′s disease (AD)-related pathologic regions. Methods： Pgc-1alphafl/fl homozygous mice were obtained by crossing Pgc-1alphafl/+ heterozygous offspring. Pgc-1alphafl/fl homozygous mice were hybridized with Cre tool mice (Calb1-Cre), which specifically expressed Cre recombinase in AD lesion brain regions (cortex and hippocampus), and the genetic DNA of progeny transgenic mice was extracted and identified by PCR. The expression of PGC-1α protein in brain and kidney of transgenic mice and wild-type mice was detected by Western blotting. Results： Dual PCR analysis revealed that mice exhibiting simultaneous amplification of 400 bp and 444 bp bands were characterized by Pgc-1alpha deletion specific to AD-related brain regions (Pgc-1alphafl/fl::Calb1-Cre, Pgc-1alpha-/-). The results of Western blotting showed that the expression of PGC-1α protein in the brain region of conditioned knockout mice was significantly decreased compared with that of wild-type mice (P<0.05). Conclusion： Conditional Pgc-1alpha knockout mice in AD-related pathological brain regions were successfully constructed.

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Received: 07 April 2023

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	SHI Houzhen1
	ZHANG Weijun1
	ZHAI Hongru1
	WANG Yijie1
	WANG Yuxin1
	LIU Zizhong2
	WANG Jia1
	2
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Cite this article:

SHI Houzhen1,ZHANG Weijun1,ZHAI Hongru1等. Construction of Pgc-1alpha knockout mouse model in Alzheimer′s disease related pathological brain regions[J]. Journal of Jiangsu University(Medicine Edition), 2024, 34(05): 423-427,450.

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2024/V34/I05/423

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