基于网络药理学和分子对接技术探讨车前子治疗高尿酸血症的作用机制

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Abstract Objective： To explore the mechanism of Plantaginis Semen (PS) in the treatment of hyperuricemia (HUA) based on network pharmacology and molecular docking technology, in order to provide ideas for the treatment of HUA. Methods： The active components of PS were determined according to reported data of previous studies, and the targets of HUA were screened in combination with TTD, OMIM and GeneCards databases, so as to determine the core targets of the intersection of ingredients and diseases. The components-target-disease network was constructed using Cytoscape software. GO functional enrichment analysis and KEGG pathway enrichment analysis were performed on the target, and the obtained results were verified by molecular docking technology and RT-qPCR technology. Results： A total of 85 common disease targets were obtained, and the core targets were CASP3, PPARG, IL-6, ESR1, etc. There were 56, 15 and 18 GO functional items obtained by GO enrichment analysis at the biological process, cytoomics and molecular function levels, respectively, and 44 pathways were obtained by KEGG pathway enrichment analysis, including TNF, metabolic signal transduction, insulin resistance, HIF-1 signaling pathway and so on. Molecular docking results showed that the binding performance between the main active ingredient and the core target was good. RT-qPCR verified the difference of Ras and p38 mRNA expression levels between the PS administration group and the model group (P<0.01). Conclusion： In this study, the "component-target-pathway" network of PS for the treatment of HUA was established, which provides a reference for systematically elucidating the molecular mechanisms of PS in treating HUA.

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Received: 22 December 2023

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	Articles by authors
	WANG Liting
	LIU Tian*
	MU Leixin
	WANG Kaihe
	KE Caiyi
	JI Li
	XU Guang
	MA Qun

Cite this article:

WANG Liting,LIU Tian*,MU Leixin等. Investigation the mechanism of Plantaginis Semen in the treatment of hyperuricemia based on network pharmacology and molecular docking[J]. Journal of Jiangsu University(Medicine Edition), 2024, 34(05): 369-376,382.

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2024/V34/I05/369

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