日本血吸虫源多肽SJMHE1通过调节脾脏IFN-γ和IL-10的分泌抑制小鼠自身免疫性甲状腺炎

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Abstract Objective： To investigate the intervention effect and mechanism of SJMHE1, a peptide derived from Schistosoma japonicum, on autoimmune thyroiditis (AIT) mice. Methods： Fifteen female C57BL/6 mice aged 6~8 weeks were randomly divided into control group, AIT model group and AIT+SJMHE1 group, with five mice in each group. Control group mice were without any treatment; the AIT mouse model (AIT model group) was established by feeding with high iodine water and subcutaneous multipoint injection of porcine thyroglobulin; and SJMHE1 was administered to AIT+SJMHE1 group. After 28 days of modeling, Hematoxylineosin (HE) staining was used to observe the pathological changes of thyroid in mice. The expression of thyroglobulin antibody (TgAb) in serum of mice was detected by chemiluminescence. The expression levels of IFNγ and IL10 mRNA in thyroid and spleen tissues of mice were detected by realtime quantitative PCR. Serum IFNγ and IL10 were detected by ELISA. Another 10 female C57BL/6 mice without any treatment were divided into experiment group and control group, with five mice in each group. FITC-labeled SJMHE1 (FITC-SJMHE1) was injected subcutaneously into mice of experimental group, and equal amount of PBS was injected into mice of control group. The distribution of FITC-SJMHE1 in thyroid and spleen tissues was detected by flow cytometry. Results： SJMHE1 significantly reduced the infiltration of inflammatory cells and damage of follicular structure in thyroid tissue of AIT mice, as well as the level of TgAb in serum (P<0.01). In addition, SJMHE1 significantly decreased IFN-γ mRNA expression and upregulated IL-10 mRNA relative level in the thyroid and spleen tissue of AIT mice (P<0.05). The results were consistent with the levels of IFN-γ and IL-10 in serum of mice (P<0.01 and P<0.05). Flow cytometry showed that FITC-SJMHE1 was only distributed in mouse spleen. Conclusion： SJMHE1 can significantly reduce thyroid pathology in AIT mice, which may be related to the regulation on IFNγ and IL10 in spleen.

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Received: 23 July 2022

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2023/V33/I01/37

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