|
|
Simultaneous determination of eight components in Prunella vulgaris L. by dual wavelength HPLC#br# |
SUN Mengqiu, SHE Shengnan, ZHOU Lihong, CHENG Jiaxi, FENG Chunlai |
(School of Pharmacy, Jiangsu University, Zhenjiang Jiangsu 212013, China)
|
|
|
Guide |
|
Abstract Objective: To establish a HPLC method for simultaneous determination of caffeic acid, pcoumaric acid, scopoletin, rosmarinic acid, esculetin, rutin, hyperin and quercetin in Prunella vulgaris L.. Methods: The separation was performed on a InertSustainTM ODSC18 column (250.0 mm×4.6 mm, 5 μm), with mobile phase consisted of acetonitrile-0.1% acetic acid solution flowing at 1.0 mL/min in a gradient elution manner. The column temperature was 30 ℃ and the detection wavelength was set at 325 nm and 360 nm. The established HPLC method was investigated by linear relationship test, precision test, stability test, repeatability test and recovery test. Results: The eight components showed good linear relationship in the range of 1-100 μg/mL, and the average recoveries were from 98.63% to 102.36%. Relative standard deviations of precision test, stability test and repeatability test were all lower than 3%. Conclusion: This simple and reliable method may be applied to accurately determine the contents of eight components, which could be used for the quality control of Prunella vulgaris L..
|
Received: 20 February 2022
|
Fund: |
|
|
|
[1]中国药典2020年版. 一部[S]. 2020: 292.
[2]严东,谢文剑,李春,等.夏枯草化学成分及其体外抗肿瘤活性研究[J].中国实验方剂学杂志, 2016, 22(11): 49-54.
[3]秦蕊, 陆军. 夏枯草的化学成分及药理作用的研究进展[J].中国医药指南, 2012, 10(36): 435-436.
[4]张金华, 邱俊娜, 王路, 等. 夏枯草化学成分及药理作用研究进展[J].中草药, 2018, 49(14): 3432-3440.
[5]崔体圣, 苗明三. 夏枯草的化学、药理及临床应用探讨[J].中医学报, 2014, 29(3): 386-388.
[6]翟雪蓓, 武晓媛, 徐晓阳, 等.高效液相色谱测定中药夏枯草活性成分含量[J].世界最新医学信息文摘, 2019, 19(69): 118.
[7]杨洁, 戚进, 余伯阳. 不同产地夏枯草中总三萜及总酚酸的含量测定[J].海峡药学, 2015, 27(8): 29-31.
[8]方罗, 林能明, 吴永江. 高效液相色谱同时测定夏枯草药材中4种活性成分的含量[J].中国中药杂志, 2010, 35(5): 616-619.
[9]黄兹英, 王继良, 黄敏洁, 等. HPLC法同时测定夏枯草中芦丁、金丝桃苷、槲皮素和山柰酚[J].中成药, 2012, 34(3): 520-523.
[10]皮胜玲, 郭小兰, 刘密, 等.野生与栽培夏枯草不同生长期3种酚酸成分含量动态研究[J].中南药学, 2021, 19(1): 100-104.
|
|
|
|