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| Expression and significance of the long noncoding RNA TMEVPG1 in peripheral blood from sepsis patients#br# |
| XU Juan1, PENG Huiyong2, CAI Yan1 |
(1. Department of Critical Care Medicine, 2. Department of Clinical Laboratory, Affiliated People′s Hospital of Jiangsu University, Zhenjiang Jiangsu 212002, China)
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Abstract Objective： To investigate the expression of long noncoding RNA TMEVPG1 in peripheral blood mononuclear cells（PBMCs) from sepsis patients and the corresponding clinical significance. Methods： A total of 31 sepsis patients admitted to the Department of Critical Care Medicine of the Affiliated People′s Hospital of Jiangsu University from January 2021 to December 2021 were selected as the sepsis group, and 32 healthy subjects as the control group. The transcript levels of TMEVPG1 and interferonγ(IFNγ) in PBMCs were detected by realtime quantitative PCR. The relationship between TMEVPG1 levels and IFN-γ mRNA levels, APACHE Ⅱ score, neutrophil to lymphocyte ratio（NLR）, and neutrophil to lymphocyte and platelet ratio(N/LPR) were analyzed. ROC curve was performed to analyze the diagnostic value of TMEVPG1 for sepsis. Results： The transcript levels of TMEVPG1 in PBMCs from sepsis patients were significantly decreased compared with the controls. There was a significantly inverse correlation between TMEVPG1 levels and APACHE Ⅱ score of sepsis patients. Meanwhile, TMEVPG1 expression also negatively correlated with NLR and N/LPR in patients with sepsis. Compared with the controls, IFN-γ mRNA expression in PBMCs from sepsis patients was downregulated, and there was a significantly positive correlation between TMEVPG1 levels and IFN-γ mRNA levels in sepsis patients. ROC curves showed that TMEVPG1 could distinguish sepsis patients from healthy controls. Conclusion： Our findings indicated that TMEVPG1 was decreased in the peripheral blood of sepsis patients and might play a pivotal role in the pathogenesis of sepsis by regulating the expression of IFN-γ.
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Received: 10 March 2022
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