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Abstract Objective: To evaluate the efficacy of the mouse model of suture-induced inflammatory corneal neovascularization and its significance. Methods: The cornea of BALB/c mice was sutured by “8” stitch with 11-0 nylon thread to establish the mouse model of suture-induced inflammatory corneal neovascularization. Lymphangiogenesis and angiogenesis were observed by slit-lamp at 3, 7, 12 days after suturing and by immunofluorescence at 5, 7, 14 days after suturing. Corneal inflammation was observed by HE staining at 3, 7, 14 days after suturing. The expression of TNF-α mRNA in inflammatory cornea was detected by qRT-PCR at 1, 3, 7, 11 and 14 days after suturing. Results: The results of slitlamp observation and immunofluorescence showed that significant lymphangiogenesis and angiogenesis were observed on the 3rd day after suturing, and the growth peaked on the 7th day after suturing. HE staining showed obvious corneal edema and inflammatory cell infiltration on the 3rd day after suturing, which began to subside on the 7th day after suturing. TNF-α mRNA expression in the sutured corneas persisted in increasing during the 7th day after suturing, and then began to decline, and kept at a low level (P<0.05). Conclusion: The mouse model of suture-induced inflammatory corneal neovascularization is a typical inflammatory model, which conforms to regular pattern of inflammation. Thus it maybe an ideal tool to explore the growth mechanism of inflammatory lymphangiogenesis.
［Key words］cornea inflammation model; mice; lymphangiogenesis; angiogenesis
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