醇沉金蝉花粗多糖对宫颈癌细胞的增殖抑制作用及可能机制

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Abstract Objective: To investigate the effect and possible mechanism of polysaccharide from Cordyceps cicadae on the proliferation of human cervical cancer Hela cells. Methods: The polysaccharides of Cordyceps cicadae were extracted by water extraction and alcohol precipitation method, and were deproteinized by Sevage method to obtain 50% and 80% alcohol precipitation CP50 and CP80. Human cervical cancer Hela cells were divided into control group （DMEM medium）, CP50 and CP80 treatment groups （25, 50, 100, 200, 400, 800 and 1 600 μg/mL） and positive control group （5fluorouracil, 50 μg/mL）. CCK8 assay was used to detect the cell activity, microscope was used to observe the morphology of Hela cells, DCFHDA probe was used to detect the level of reactive oxygen species in Hela cells, realtime fluorescence quantitative PCR was used to detect the mRNA expression of BAX, BCL2 and P53 in Hela cells, and Western blotting was used to detect the protein expression of BAX, BCL2 and P53 in Hela cells. Results: Compared with the control group, CP50 and CP80 significantly inhibited the proliferation of Hela cells in a concentrationdependent manner. Compared with the control group, the reactive oxygen activity in Hela cells treated with CP80 was significantly increased （P<0.05）, the gene and protein expression levels of BAX and P53 were significantly increased （P<0.05）, and the gene and protein expression levels of BCL2 were significantly decreased （P<0.05）. Conclusion: Polysaccharides from Cordyceps cicadae have significant inhibitory effect on Hela cells, and the mechanism may be increasing reactive oxygen activity in Hela cells, regulating the expression of BAX and BCL2 genes and proteins, and mediating the apoptosis of Hela cells through the P53 signaling pathway.

Received: 02 December 2020

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	Articles by authors
	LU.. Wanlin
	ZHOU Hongqiu
	ZHU Yiling
	WANG Yishu
	WEI Yuan
	OUYANG Zhen

Cite this article:

LU.. Wanlin,ZHOU Hongqiu,ZHU Yiling等.

Inhibitory effect and mechanism of crude polysaccharide from Cordyceps cicadae on proliferation of cervical cancer cells

[J]. Journal of Jiangsu University(Medicine Edition), 2021, 31(02): 152-157.

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2021/V31/I02/152

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