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Protection of CO-releasing molecules-2 against the random narrow pedicleskin flap with ischemia-reperfusion injury in rats |
| GAO Liang, TAI Ning-zheng |
(Department of Burns and Plastic Surgery,the Affiliated Hospital of Jiangsu University,Zhenjiang Jiangsu 212001,China) |
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Abstract Objective: To observe the effect of CO-releasing molecules-2（CORM-2） on the random narrow pedicle skin flap in the SD rats with ischemia-reperfusion injury(IRI)，and discuss the possible mechanism. Methods: Established IRI models of random narrow pedicle skin flap in rats.A total of 36 SD rats were divided into two groups at random：CORM-2 group，models of random narrow pedicle skin flap and treated by CORM-2；NS group，models of random narrow pedicle skin flap and treated by normal saline(NS).Test the activity of NF-κ B by Western blotting. The content of malonaldehyde(MDA) in tissue of skin flap was detected by phenobarbitone acid；the activity of superoxide dismutase(SOD) in tissue of skin flap was detected by xanthine oxidase；the level of IL-6 and tumor necrosis factor-α(TNF-α) in blood serum was detected by ELISA；pathological changes were observed with light microscope in the 3rd, 5th, 7th day after operation；survival rate of skin flap was the average survival length of skin flap deciding practical length of skin flap. Results: ① The skin flap uncovered by the 3rd, 5th, 7th day，compared with NS group，the content of MDA in the skin flap of CORM-2 group lower(P<0.01)，the activity of SOD significantly increased (P<0.01). ② The skin flap uncovered by 3rd, 5th, 7th day，compared with NS group，the level of IL-6 and TNF-α in blood serum lower(P<0.01). ③ Histopathology showed that abundant capillary were noticed by application of CORM-2. ④ CORM-2 can effectively restrain the NF-κ B activation in random narrow pedicle skin flap model. ⑤ The skin flap was uncovered by the 7th day，compared with NS group，survival rate of skin flap of CORM2 group significantly higher(P<0.01). Conclusion: CORM-2 effectively reduced the narrow pedicle skin flap of rats with IRI, and improved the survival rate of flap.
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Received: 21 January 2014
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| [1]赵天兰，程新德，熊绍虎，等.窄蒂皮侧颌颈部皮瓣的解剖与临床应用\[J\].中华显微外科杂志，2002，25（4）：201-202.［2］赵天兰，余道江，吴浩荣，等.一定长宽比狭长窄蒂与任意皮瓣成活面积关系的实验研究\[J\].中华显微外科杂志，2009，32 (5)：393-395.［3］赵天兰，余道江，吴浩荣，等.猪狭长窄蒂随意型皮瓣蒂部不同长宽比例与成活面积关系的研究\[J\].中华整形外科杂志，2011，27(1)：40-43.［4］Zhang X，Shan P，Alam J，et al. Carbon monoxide modulates Fas/Fas ligand, caspases, and Bcl-2 family proteins via the p38α mitogen-activated protein kinase pathway during ischemia-reperfusion lung injury\[J\].J Biol Chem，2003，278(24)：22061-22070.［5］Song R，Kubo M，Morse D，et al. Carbon monoxide induces cytoprotection in rat orthotopic lung transplantation via anti-inflammatory and anti-apoptotic effects\[J\].Am J Pathol，2003,163(1)：231-242.［6］Nakao A，Choi AM，Murase N. Protective effect of carbon monoxide in transplantation\[J\]. J Cell Mol Med,2006，10(3)：650-671.［7］王法刚，薛文君.皮下注射血管内皮生长因子基因提高大鼠背部随意皮瓣活力的实验研究\[J\].中华整形外科杂志，2002，18(3)：157-159.［8］成红兵，侍德.皮瓣血供的实验研究\[J\].南通大学学报:医学版,1984,4(1)：16-19.［9］郭树忠，汪良能，鲁开化.自由基对缺血游离皮瓣的损伤和超氧化物歧化酶抗损伤作用的实验研究\[J\].中国修复重建外科杂志，1995，9(2)：104-107.［10］杜佳梅,金建学,张松林,等.一氧化氮对任意型皮瓣成活的影响\[J\].中华整形外科杂志,2002,18(6):353-356.［11］周力纯,陕声国,张瑞莲,等.一氧化氮对任意型皮瓣成活的实验研究\[J\].中华整形外科杂志,2004,20(1):38-40.［12］Inaji M，Tomita H，Tone O，et al. Chronological changes of perihematomal edema of human intracerebral hematoma\[J\].Acta Neurochir Suppl,2003,86：445-448.［13］Inagawa T，Ohhayshi N，Takechi A，et al. Primary intracerebral hemorrhage in lzumo City，Japan：incidence rates and outcome in relation to the site of hemorrhage\[J\]. Neurosurgery，2003，53（6）：1283-1297.［14］del Zoppo GJ. Microvascular responses to cerebral ischemia/inflammation\[J\].Ann N Y Acad Sci,1997，823：132-147.［15］Stannimirovic D，Shapiro A，Wong J，et al.The induction of ICAM-1 in human cerebralbromivascular endothelial cells(HCEC) by ischemia-like conditions promotes enhanced neutrophil/HCEC adhesion\[J\].J Neuroimmunol，1997，76(1/2)：193-205.［16］孙炳伟，陈曦，Kazuhiro Katada,等.外源性一氧化碳释放分子抑制脓毒症炎症反应的实验研究\[J\].江苏大学学报:医学版，2008，18（5）：369-377. |
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2014, Vol. 24 
