基质细胞衍生因子-1α对间充质干细胞迁移的影响

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Abstract Objective: To investigate the effect of stromal cell-derived factor-1α (SDF-1α) on the migration of mesenchymal stem cells (MSCs) and the mechanism involved in cell migration. Methods: MSCs were isolated from rat bone marrow in vitro. Boyden chamber was used to study the behavior of cell migration toward SDF-1α at different concentrations (0, 5, 25, 50, and 100 ng/mL). Then, we detected the phosphorylation of PI3K/Akt and MAPKs by addition of SDF1α by Western blot. Finally, pretreatment with specific chemical inhibitors to block PI3K/Akt or MAPKs signaling，we analyzed the influence of these signaling on SDF-1α-induced cell migration by Boyden chamber. Results: SDF-1α at different concentrations influenced MSCs migration by the regulation of PI3K/Akt and MAPKs signaling, and low concentrations of SDF-1α were positive related to cell migration, while high concentrations played converse effect. Interference with basal PI3K/Akt or MAPKs signaling decreased SDF-1α-induced MSCs migration to a variable extent. Conclusion: SDF-1α-induced MSCs migration was positively regulated to the changing of PI3K/Akt signaling. Meanwhile, blocking JNK/SAPK signaling significantly decreased SDF-1α-induced directed migration of MSCs. Treatment with SDF-1α could promote ERK1/2 and p38MAPK signaling pathways in MSCs, yet interference with the two signaling had no significant inhibition of cell migration.

Received: 03 June 2014

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2014/V24/I04/277

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