稳定表达狂犬病毒糖蛋白的重组新城疫病毒对人肺腺癌A549细胞荷瘤鼠瘤体生长的影响

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Abstract Objective: To explore the effects of recombinant avirulent newcastle disease virus LaSota strain expressing the rabies virus glycoprotein (rl-RVG) on the proliferation of A549 lung adenocarcinoma tumor-bearing mice and potential immune mechanism. Methods: A tumor model of human A549 into nude mice was constructed and divided randomly into rl-RVG group, newcastle disease virus(NDV) group and control group. The mice in each group received rlRVG, NDV and PBS injection, respectively, twice a week, for 3 weeks. The growth of tumor were recorded, and the expression of NDV and rabies virus glycoprotein (RVG) were assayed by immunohistochemistry. The pathological change of the tissues were observed by HE staining. The number of NK (CD3-CD49+) cells were detected by flow cytometry. Results: Compared with the control group, the growth of tumor in rl-RVG group and NDV group were inhibited more effectively. The subcutaneous tumor necrosis were more evident and there were much more multinucleated giant cells in the spleen tissue of the mice in rlRVG group and NDV group. Immunohistochemical analysis showed that RVG protein was expressed in rl-RVG group and NDV protein was expressed in both rl-RVG group and NDV group. The number of NK cells was significantly higher in rl-RVG group and NDV group than that in the control group, and in rl-RVG group it was more higher than that in NDV group. Conclusion: Recombinant rl-RVG transfected successfully, and effectively inhibited the growth of A549 lung adenocarcinoma by mechanism which activating cell immune response.

Received: 25 November 2013

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