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| Protecting effect of endothelial progenitor cell transplantation on renal injury with severe acute pancreatitis |
| DANG Shengchun, WANG Pingjiang, ZENG Yanhua, CHEN Rongfang,WANG Hao, FENG Shu, CUI Lei, ZHANG Jianxin |
| Department of General Surgery, the Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001, China |
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Abstract Objective: To explore the protecting effect of endothelial progenitor cells(EPCs) transplantation on renal complicated injury with severe acute pancreatitis(SAP). Methods: To manufacture the superparamagnetic iron oxide(SPIO) by the coprecipitation method; collecting rat EPCs from bone marrow, mononuclear cells were isolated with ficoll,VEGF, fibroblast growth factor (FGF) in the culture medium;EPCs were labeled by the SPIO; 72 rats were randomly divided into control group, SAP group, SPIOEPCs group. The SAP models were established by injection of sodium taurocholate into the pancreatic capsule. Rats in the SPIOEPCs group were injected with SPIOEPCs through tail vein. Rats in SAP group and control group only received phosphate buffered solution with the same dose. Then the serum levels of AMS, BUN, Cr and TNFα at 2 h, 6 h,12 h after modeling. Pathologic alteration of kidney were also observed. HE staining was used to observe the pathologic change of kidney tissue on different time between three groups. Prussian blue staining was used to observe the change of iron particles in SPIOEPCs group on different time. Results: In SAP group serum levels of AMS, BUN, Cr and TNFα were significantly elevated compared with control group (P<0.01). Compared with SAP group, serum levels of AMS, BUN, Cr and TNFα were significantly decreased in SPIOEPCs group(P<0.01). The pathological scores of renal in SAP group were higher than those of control group (P<0.05). The pathological scores of renal in SPIOEPCs group were lower than those of SAP group(P<0.05). From the Prussian blue staining, we can observe that the iron particles in renal vascular endothelial increased gradually on the time passed. Conclusion: EPCs transplantation can reduce the release of inflammatory mediators, reduce renal vascular endothelial injury SAP . The transplanting EPCs can alleviate the renal injury with SAP.
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Received: 12 November 2013
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2014, Vol. 24 
