Abstract：[Abstract] Objective: To observe the impact of hydrogen-rich saline on the senescence of kidney in aging rats. Methods: Ten healthy adult male Sprague-Dawley rats aged 3-4 months were selected in the study as the adult group; meanwhile, twenty healthy male SD rats aged 1820 months were randomized into aging group and hydrogen-rich saline group. Adult group and aging group rats received intragastric administration of saline(5 mL·kg-1·d-1), while hydrogenrich saline group rats received hydrogen-rich saline(5 mL·kg-1·d-1).The other conditions were the same in the three groups. After eight weeks, the superoxide dismutase(SOD), malondialdehyde(MDA), 8-hydroxy-2′-deoxyguanosine(8-OHdG) contents in kidney tissues from all the rats were determined; and the mRNA levels of p53, p16, SIRT1 were measured by RT-PCR. Results: Aging group had greatly lower SOD contents, higher MDA and 8-OHdG concentrations than adult group; while hydrogen-rich saline group had significantly higher SOD contents, lower MDA and 8-OHdG concentrations than aging group(P<0.01 or P<0.05); Compared with adult group, aging group had significantly higher p53 and p16 mRNA levels with lower SIRT1 mRNA levels (P<0.01 or P<0.05); on the contrary, hydrogen-rich saline group had markedly lower p53 and p16 mRNA levels with higher SIRT1 mRNA levels, compared with aging group(both P<0.01). Conclusion: Hydrogen-ich saline could play a role in delaying kidney senescence, which was probably related to the inhibition of p53 and p16 expression and the improvement of SIRT1 expression.
收稿日期: 2013-04-23
基金资助:
江苏省生命健康科技专项资金资助项目(BL2012045)
通讯作者:
何小舟,教授,博士生导师,E-mail:wypyxl050508@163.com
作者简介: 袁晓亮(1985—),男,硕士研究生;
引用本文:
袁晓亮, 何小舟, 许贤林, 范敏. 饱和氢气盐溶液对高龄大鼠肾脏衰老的影响[J]. 江苏大学学报:医学版, 2013, 23(4): 296-299，303.
YUAN Xiao-liang, HE Xiao-zhou, XU Xian-lin, FAN Min.
Influence of hydrogenrich saline on senescence in aging rat kidney. Journal of Jiangsu University(Medicine Edition), 2013, 23(4): 296-299，303.
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