MDM2环状RNA在急性髓系白血病患者骨髓中的表达及临床意义" />
MDM2环状RNA在急性髓系白血病患者骨髓中的表达及临床意义" />
Expression and clinical significance of MDM2 circular RNA in bone marrow of patients with acute myeloid leukemia" />
Expression and clinical significance of MDM2 circular RNA in bone marrow of patients with acute myeloid leukemia
ZHAO Qian 1,2, WU Delong 1,2, SU Xiaoyu 1,2, ZHU Xin 1,2, CHU Mingqiang 1,2, LIN Jiang 1,2, DENG Zhaoqun 1,2
(1. Department of Laboratory Center, Affiliated People′s Hospital of Jiangsu University, Zhenjiang Jiangsu 212002; 2. the Key Lab of Precision Diagnosis and Treatment of Zhenjiang City, Zhenjiang Jiangsu 212002, China)
Objective: To explore the expression level and potential clinical significance of murine double minute 2 (MDM2) circular RNA (circ-MDM2) in bone marrow specimens of patients with acute myeloid leukemia (AML). Methods: Expression level of circMDM2 in bone marrow mononuclear cells (BMMNCs) of 92 AML patients and 22 controls was detected by quantitative real-time PCR. Receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value of circ-MDM2 expression. The association between the expression level of circ-MDM2 and the clinical parameters of AML patients was analyzed. Kaplan-Meier curve was used to analyze the influence of circ-MDM2 on overall survival and leukemia-free survival. Results: Compared with the control group, circ-MDM2 was significantly increased in AML patients (P<0.05). According to the ROC curve result, the area under curve of circ-MDM2 for diagnosing AML was 0.649, and the expression level of circ-MDM2 could distinguish AML patients from controls (P=0.03). Mutation rate of CEBPA in circ-MDM2 low expression group was significantly higher than that in circ-MDM2 high expression group (P=0.039). Overall survival and leukemia-free survival of patients in high-expressed circ-MDM2 group and low-expressed circ-MDM2 group were not statistically significant (P>0.05). Conclusion: circ-MDM2 was significantly up-regulated in AML patients and could be used as a potential biomarker for the diagnosis of AML.