大蒜活性成分二烯丙基三硫化物调控Wnt通路抑制香烟烟雾增强的胃癌干细胞干性

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摘要目的: 探讨大蒜活性成分二烯丙基三硫化物（diallyl trisulfide, DATS）能否干预香烟烟雾促进的SGC7901源胃癌干细胞干性，并探究Wnt通路在其中的作用。方法: 用不同浓度（0%、0.25%和0.50%）的香烟烟雾悬液和DATS单独或联合处理SGC7901源胃癌干细胞7 d，显微镜下观察胃癌干细胞球大小及数量的变化，实时定量PCR及蛋白质印迹检测干性基因NANOG、OCT4、SOX2、LIN28的mRNA和蛋白表达；香烟烟雾悬液、DATS和氯化锂单独或联合处理胃癌干细胞，蛋白质印迹检测干性基因及Wnt通路相关蛋白表达的变化。结果： DATS能明显抑制香烟烟雾促进的胃癌干细胞球的形成及干性基因NANOG、OCT4、SOX2、LIN28的mRNA和蛋白表达；蛋白质印迹结果显示，DATS能抑制香烟烟雾诱导的β连环蛋白、糖原合成酶激酶 3β（GSK3β）表达异常；氯化锂能增强NANOG和OCT4的表达，与对照组相比，DATS和氯化锂联合处理组GSK3β蛋白表达水平下调，而β连环蛋白表达水平上调，同时NANOG和OCT4的蛋白表达水平上调。结论： DATS能抑制香烟烟雾促进的SGC7901源胃癌干细胞干性，Wnt通路在其中发挥重要作用。

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	郭文浩
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关键词 ：胃癌干细胞, 香烟烟雾, 二烯丙基三硫化物, Wnt通路

Abstract：Objective: To investigate whether diallyl trisulfide （DATS） reverse the stemness of SGC-7901-derived gastric cancer stem cells promoted by cigarette smoke, and explore the role of Wnt pathway. Methods: The gastric cancer stem cells obtained from SGC-7901 cells were treated with different concentrations of cigarette smoke extract （CSE, 0, 0.25% and 0.50%） and DATS alone or in combination for 7 days. The size and number of gastric cancer stem cell spheres under microscope were observed. Real-time quantitative PCR and Western blotting were used to detect the expression of stemness genes NANOG, OCT-4, SOX2 and LIN28. CSE, DATS and Licl were used alone or in combination to treat gastric cancer stem cells. Western blotting were used to detect the expression of stemness genes and Wnt pathway. Results: Compared with CSE alone groups, DATS significantly inhibited the formation of gastric cancer stem cell spheres promoted by CSE and the expression of NANOG, OCT-4, SOX2 and LIN28；Western blotting results showed the abnormal expression of β-catenin and GSK3β protein levels caused by CSE were inhibited. LiCl could enhance the expression of stemness genes NANOG and OCT-4. Compared with the control group, the expression of GSK3β protein level in DATS and LiCl group was down-regulated, while the expression of β-catenin protein level was up-regulated; the expression of NANOG and OCT-4 protein levels were up-regulated. Conclusion: DATS inhibited the stemness of SGC-7901-derived gastric cancer stem cells promoted by cigarette smoke, which may attribute to regulation of the Wnt pathway.

收稿日期: 2020-08-10

基金资助:江苏省研究生科研与实践创新计划项目（KYCX20_3089）；江苏大学“青年英才培育计划”优秀青年骨干教师培育项目；江苏大学实验动物中心大学生科研创新资助项目

通讯作者: 梁照锋（通讯作者），副教授，硕士生导师，E-mail：liangzhaofeng@ujs.edu.cn

作者简介: 郭文浩（1995—），男，硕士研究生

引用本文:

郭文浩，房士琨，张玥，梁照锋. 大蒜活性成分二烯丙基三硫化物调控Wnt通路抑制香烟烟雾增强的胃癌干细胞干性[J]. 江苏大学学报:医学版, 2021, 31(02): 129-133. GUO Wenhao, FANG Shikun, ZHANG Yue, LIANG Zhaofeng.

Diallyl trisulfide reversed cigarette smokeenhanced stemness of SGC-7901-derived gastric cancer stem cells via Wnt pathway

. Journal of Jiangsu University(Medicine Edition), 2021, 31(02): 129-133.

链接本文:

http://zzs.ujs.edu.cn/xbyxb/CN/ 或 http://zzs.ujs.edu.cn/xbyxb/CN/Y2021/V31/I02/129

［1］Yoshida K, Gowers K, Henry LS, et al. Tobacco smoking and somatic mutations in human bronchial epithelium\[J\]. Nature, 2020,578（7794）:266-272.

［2］Praud D, Rota M, Pelucchi C, et al. Cigarette smoking and gastric cancer in the Stomach Cancer Pooling （StoP） Project\[J\]. Eur J Cancer Prev，2018，27（2）：124-133.

［3］Ruggieri V, Russi S, Zoppoli P, et al. The role of microRNAs in the regulation of gastric cancer stem cells: a metaanalysis of the current status\[J\]. J Clin Med, 2019, 8（5）:639.

［4］梁照锋,陶海,郭文浩, 等.香烟烟雾促进膀胱癌干细胞的自我更新和干性基因的表达\[J\].江苏大学学报（医学版）,2019,29（3）:205-207.

［5］Haghi A, Azimi H, Rahimi R. A comprehensive review on pharmacotherapeutics of three phytochemicals, curcumin, quercetin, and allicin, in the treatment of gastric cancer\[J\]. J Gastrointest Cancer，2017，48（4）：314-320.

［6］Zheng JX, Cheng X, Xu SC, et al. Diallyl trisulfide induces G2/M cellcycle arrest and apoptosis in anaplastic thyroid carcinoma 8505C cells\[J\]. Food Funct, 2019, 10（11）:7253-7261.

［7］Qian W, Kong X, Zhang T, et al. Cigarette smoke stimulates the stemness of renal cancer stem cells via Sonic Hedgehog pathway\[J\]. Oncogenesis，2018，7（3）：24.

［8］Wang J, Chen J, Jiang Y, et al. Wnt/βcatenin modulates chronic tobacco smoke exposureinduced acquisition of pulmonary cancer stem cell properties and diallyl trisulfide intervention\[J\]. Toxicol Lett，2018，291：70-76.

［9］Nimmakayala RK, Seshacharyulu P, Lakshmanan I, et al. Cigarette smoke induces stem cell features of pancreatic cancer cells via PAF1\[J\]. Gastroenterology，2018，155（3）：892-908. e6.

［10］Tao M, Gao L, Pan J, et al. Study on the inhibitory effect of allicin on human gastric cancer cell line SGC7901 and its mechanism\[J\]. Afr J Tradit Complement Altern Med，2014，11（1）：176-179.

［11］Liu Y, Zhao Y, Wei Z, et al. Targeting thioredoxin system with an organosulfur compound, diallyl trisulfide （DATS）, attenuates progression and metastasis of triplenegative breast cancer （TNBC）\[J\]. Cell Physiol Biochem，2018，50（5）：1945-1963.